CAJ | 학술논문

建立了用毛细管电泳间接紫外检测法分析人发中多种微量金属离子的方法. 考察了缓冲液种类,检测波长,缓冲液浓度,缓冲液pH,分离电压及柱温对分离的影响. 采用浓度为20 mmol·L-1的咪唑溶液(乙酸调节pH=4. 0)作为背景电解液,压力进样(50 mbar × 5 s),分离电压15 kV,检测波长214 nm,柱温25 ℃实验条件下,在7 min内实现了Ca2+、Mg2+、Fe2+、Zn2+、Cu2+5种金属离子的分离与测定. 方法的线性范围为0. 25~100. 00 mg·L-1 ,检测限为0. 10~1. 00 mg·L-1 ,重复5次测定保留时间的相对标准偏差( RSD)小于0. 5%,峰面积的相对标准偏差( RSD)小于2. 6%. 加标回收实验结果较好,回收率为94. 3% ~104. 0%. 该方法简单快速,重复性好,准确度高,应用于人发样品的分析,测定结果准确,所测出的金属离子与电感耦合等离子体原子发射光谱法( ICP-AES)测定结果无显著性差异.
건립료용모세관전영간접자외검측법분석인발중다충미량금속리자적방법. 고찰료완충액충류,검측파장,완충액농도,완충액pH,분리전압급주온대분리적영향. 채용농도위20 mmol·L-1적미서용액(을산조절pH=4. 0)작위배경전해액,압력진양(50 mbar × 5 s),분리전압15 kV,검측파장214 nm,주온25 ℃실험조건하,재7 min내실현료Ca2+、Mg2+、Fe2+、Zn2+、Cu2+5충금속리자적분리여측정. 방법적선성범위위0. 25~100. 00 mg·L-1 ,검측한위0. 10~1. 00 mg·L-1 ,중복5차측정보류시간적상대표준편차( RSD)소우0. 5%,봉면적적상대표준편차( RSD)소우2. 6%. 가표회수실험결과교호,회수솔위94. 3% ~104. 0%. 해방법간단쾌속,중복성호,준학도고,응용우인발양품적분석,측정결과준학,소측출적금속리자여전감우합등리자체원자발사광보법( ICP-AES)측정결과무현저성차이.
A method was developed for the determination of trace metal ions in human hair by capillary electrophoresis. The effect factors such as the buffer type, detection wavelength, buffer concentration, buffer pH, separation voltage and column temperature were investigated. Five metal ions of Ca2+, Mg2+, Fe2+, Zn2+, Cu2+ were separated and determination within 7 min utilizing the concentration of 20 mmol/L imidazole (pH adjusted to 4. 0 by acetic acid) as the background electrolyte solution and the pressure injection (50 mba × 5 s),the separation voltage of 15 kV,the detection wavelength was set at 214 nm and the column temperature was 25 ℃. The linear range ranged from 0. 25 to 100. 00 mg/L, the detection limit ranged from 0. 10 to 1. 00 mg/L. Determination of repeat 5 times, the relative standard deviation (RSD) of retention time was less than 0. 5%, the relative standard deviation (RSD) of peak area was less than 2. 6%. The results of spiked recovery were good and the recoveries were obtained from 94. 3% to 104. 0%.The method was simple, fast, reproducible and high accuracy. The determination resultsofmetal ionswere accurate and had no significant difference compared with inductively coupled plasma atomic emission spectrometry ( ICP-AES) when applied to the human hair sample analysis.