CAJ | 학술논문

利用NCBI在线数字化差异显示工具 ( Digital Differential Display) 对笠贝( Lottia gigantea) EST数据库进行筛选, 获得笠贝幼虫时期显著差异表达基因203个, 其中124个基因有Blast2Go注释结果, 这124个基因可以归类成细胞组分、分子功能和生物学过程三大类; 参与MAPK、 Ras、 PI3K-Akt、 Rap1、cAMP等信号通路; 涉及能量代谢、免疫应激、细胞分化、神经发育、细胞骨架、信号传递等生物学过程.将获得的转录子运用Cytoscape软件构建分子相互作用网络, 最终获得7个核心转录子. 涉及到神经发育、次生壳形成等相关基因调控关系.
이용NCBI재선수자화차이현시공구 ( Digital Differential Display) 대립패( Lottia gigantea) EST수거고진행사선, 획득립패유충시기현저차이표체기인203개, 기중124개기인유Blast2Go주석결과, 저124개기인가이귀류성세포조분、 분자공능화생물학과정삼대류; 삼여MAPK、 Ras、 PI3K-Akt、 Rap1、cAMP등신호통로; 섭급능량대사、 면역응격、 세포분화、 신경발육、 세포골가、 신호전체등생물학과정.장획득적전록자운용Cytoscape연건구건분자상호작용망락, 최종획득7개핵심전록자. 섭급도신경발육、차생각형성등상관기인조공관계.
By using the NCBI online Digital Differential Display tool, 203 differentially expressed genes in metamorphosis of Lottia gigantea larvae were identified from ESTs database of L. gigantea . About 124 genes had Blast2Go annotations which were classified into cellular components, molecular functions and bio-logical processes by GO ( Gene Ontology ) . These genes are members of some signal pathways, such as MAPK, Ras, PI3K-Akt, Rap1, cAMP, et al, and refer to many biological processes, such as energy me-tabolism, immunological stress, cell differentiation, neurodevelopment, cytoskeleton, signal transmission. These 124 genes were used to build molecular interaction network by Cytoscape software. A core network, in-cluding seven core genes, was showed. The network involved in nerve development, tissue differentiation, secondary shell formation and energy metabolism, and revealed a possible molecular mechanism of metamor-phosis.