CAJ | 학술논문

为了研究大黄鱼源变形假单胞菌胞外产物的致病性, 在内脏白点病的高发温度 ( 18 ℃) 培养大黄鱼的病原菌——变形假单胞菌, 利用玻璃纸覆盖平板技术制备其胞外产物, 测定不同温度 (低温不致病温度12℃、高发致病温度18℃和高温不致病温度28℃) 下其胞外产物的酶活力. 结果显示: 变形假单胞菌胞外产物的淀粉酶、丝氨酸蛋白酶类、天冬氨酸蛋白酶类、类胃蛋白酶、类糜蛋白酶、卵磷脂酶、酸性磷酸酯酶和碱性磷酸酯酶的活性受温度影响比较大, 28 ℃条件下酶活力显著(P < 0. 05)高于12 ℃或者18 ℃时的酶活力; 半胱氨酸蛋白酶类、氨肽酶的活性和溶血活性受温度影响没有显著性变化(P > 0. 05) .实验结果表明, 虽然变形假单胞菌胞外产物是其致病因素之一, 但大黄鱼内脏白点病在特定温度 ( 16 ~20 ℃) 下高发并非是由于胞外产物在该温度下的活性较高. 该结果增进了对大黄鱼内脏白点病发病机理的认识.
위료연구대황어원변형가단포균포외산물적치병성, 재내장백점병적고발온도 ( 18 ℃) 배양대황어적병원균——변형가단포균, 이용파리지복개평판기술제비기포외산물, 측정불동온도 (저온불치병온도12℃、 고발치병온도18℃화고온불치병온도28℃) 하기포외산물적매활력. 결과현시: 변형가단포균포외산물적정분매、 사안산단백매류、 천동안산단백매류、 류위단백매、 류미단백매、 란린지매、 산성린산지매화감성린산지매적활성수온도영향비교대, 28 ℃조건하매활력현저(P < 0. 05)고우12 ℃혹자18 ℃시적매활력; 반광안산단백매류、 안태매적활성화용혈활성수온도영향몰유현저성변화(P > 0. 05) .실험결과표명, 수연변형가단포균포외산물시기치병인소지일, 단대황어내장백점병재특정온도 ( 16 ~20 ℃) 하고발병비시유우포외산물재해온도하적활성교고. 해결과증진료대대황어내장백점병발병궤리적인식.
In order to study the pathogenicity of ECP from Peseudomonas plecoglassicida , the P. plecoglassicida were cultured at high pathogenic temperature and its ECP was prepared by the cellophane plate technique. The enzyme activities of ECP were measured at different temperatures ( hypothermy nonpathogenic temperature 12 ℃, high pathogenic temperature 18 ℃ and hyperthermy nonpathogenic temperature 28 ℃) . The results showed that the activities of amylase, serine proteinase, aspartate proteinase, pepsinase-like, chymotrpsin-like, lecithinase, acid phosphatase and alkaline phosphatase of ECP of P. plecoglassicida were affected by temperature notably, and at 28 ℃ their activities were significantly higher than at 12 ℃ or 18 ℃(P < 0. 05) while the enzymatic activities of cysteine proteinase, aminopeptidase and hemolysis activity were not significantly changed (P >0. 05) . The results of this paper revealed that P. croceavisceral white spot dis- ease had high incidence at specific temperature interval (16~20℃) not because ECP possess higher activity at this temperature interval although the ECP is one of the pathogenic foctors. The research results of this pa-per could improve the understanding of pathogenesis of P. crocea visceral white spot disease.