CAJ | 학술논문

应用PCR及RACE技术从日本鳗鲡( Anguilla japonica)中克隆获得一个2463 bp环氧化酶基因(Cyclooxygenase-2,AjCOX-2) 的cDNA序列. AjCOX-2前体为606肽, 含有脊椎动物COX-2的保守结构域,以及加氧酶和过氧化物酶的活性位点. 在正常生理条件下, AjCOX-2基因在日本鳗鲡的鳃、鳔和皮肤中高水平转录表达. 脂多糖刺激后8 h, AjCOX-2基因在皮肤和鳔中的转录表达显著上调(P < 0. 05) ; 多聚胞苷酸刺激后 8 h, AjCOX-2 在皮肤、鳔和鳃中的转录表达显著上调 (P < 0. 05) ; 迟缓爱德华氏菌(Edwardsiella tarda) 刺激后 8 h, AjCOX-2 在鳔中的转录表达也极显著上调 (P < 0. 01). 结果表明, AjCOX-2基因是对外来病原刺激的早期响应基因, 参与机体抵抗外来刺激物的先天免疫应答.
응용PCR급RACE기술종일본만려( Anguilla japonica)중극륭획득일개2463 bp배양화매기인(Cyclooxygenase-2,AjCOX-2) 적cDNA서렬. AjCOX-2전체위606태, 함유척추동물COX-2적보수결구역,이급가양매화과양화물매적활성위점. 재정상생리조건하, AjCOX-2기인재일본만려적새、 표화피부중고수평전록표체. 지다당자격후8 h, AjCOX-2기인재피부화표중적전록표체현저상조(P < 0. 05) ; 다취포감산자격후 8 h, AjCOX-2 재피부、 표화새중적전록표체현저상조 (P < 0. 05) ; 지완애덕화씨균(Edwardsiella tarda) 자격후 8 h, AjCOX-2 재표중적전록표체야겁현저상조 (P < 0. 01). 결과표명, AjCOX-2기인시대외래병원자격적조기향응기인, 삼여궤체저항외래자격물적선천면역응답.
In this study, a full-length cDNA for Cyclooxygenase-2(AjCOX-2) was successfully cloned from the Japanese eel ( Anguilla japonica) by PCR and RACE. The predicted protein of AjCOX-2 is 606 ami-no acids in length, which contained all the conservative structural domains and the active sites for both the peroxidase and cyclooxygenase as in its vertebrate homologues. In physiological condition, AjCOX-2 mRNA was highly expressed in gill, skin and swim bladder in Japanese eel. Additionally, a significant increasing AjCOX-2 mRNA was detected in both skin and swim bladder ( P < 0 . 05 ) at 8 h after in vivo intraperitoneal challenged with LPS and Poly I:C, whilst a significant up-regulation of AjCOX-2 mRNA was found only in swim bladder at 8 h after challenged by Edwardsiella tarda, a pathogenic bacterium isolated from Japanese eel. The results suggested that AjCOX-2 might be an early immediate gene in response to the immune stimuli and involved in the innate immune response against the pathogens infection in Japanese eel.