CAJ | 학술논문

以棘孢曲霉为菌种, 以磷酸氢二铵为氮源固态发酵柚皮生产柚苷酶, 结果表明, 在以柚皮为碳源和磷酸氢二铵为氮源的发酵体系中, 添加疏松剂和豆饼粉对柚苷酶发酵没有显著影响, 而磷酸氢二铵添加量和水分质量分数对柚苷酶合成具有显著影响. 当无水柚皮粉中磷酸氢二铵和水分质量分数分别为324%和1700%时,有利于提高柚苷酶发酵活力. 在接种量为71%、发酵温度为30℃的情况下发酵6 d, 柚苷酶比合成速率与棘孢曲霉的生长速率符合模型Y柚苷酶 = 62677 X -0. 0381 , 其中Y代表柚苷酶的比合成速率, X代表比生长速率. 用Davis法测得柚苷酶活力为9461 IU/g, 酶发酵的培养基成本 (5 × 10-5元/IU) 远远低于其他同类研究, 酶的纯度远高于用豆饼粉为氮源所获得的酶纯度.
이극포곡매위균충, 이린산경이안위담원고태발효유피생산유감매, 결과표명, 재이유피위탄원화린산경이안위담원적발효체계중, 첨가소송제화두병분대유감매발효몰유현저영향, 이린산경이안첨가량화수분질량분수대유감매합성구유현저영향. 당무수유피분중린산경이안화수분질량분수분별위324%화1700%시,유리우제고유감매발효활력. 재접충량위71%、 발효온도위30℃적정황하발효6 d, 유감매비합성속솔여극포곡매적생장속솔부합모형Y유감매 = 62677 X -0. 0381 , 기중Y대표유감매적비합성속솔, X대표비생장속솔. 용Davis법측득유감매활력위9461 IU/g, 매발효적배양기성본 (5 × 10-5원/IU) 원원저우기타동류연구, 매적순도원고우용두병분위담원소획득적매순도.
The naringinase production from Aspergillus aculeatus was investigated using ( NH4 ) 2 HPO4 as nitrogen source in pomelo peel fermentation. The results showed that naringinase activity did not significantly increase by adding loosening agents and soybean meal powder, while the content of diammonium hydrogen phosphate and water had significant influence on naringinase production. The composition of the optimal medi-um included anhydrous citrus peel powder, the percentage rates for other ingredients in anhydrous citrus peel powder were as follow: diammonium hydrogen phosphate, 324%; water, 1700%. By using the fermen-tation condition of inoculum size 71% and fermentation temperature 30 ℃, the naringinase fermentation was estimated to have a naringinase synthesis model Ynaringinase =62677 X-0. 0381 , where Y represented the en-zymatic specific synthetic rate and X was the specific growth rate. After fermentation for 6 days, naringinase activity attained 9461 IU/g by Davis method analysis, higher than most research before reported. In addi- tion, the medium cost was merely 5 × 10 -5 Yuan/IU naringinase, much lower than those previous studies. Furthermore, the enzyme extract revealed higher purity than that in our previous study.