CAJ | 학술논문

为探索杏鲍菇原生质体的再生条件，探讨了再生方式、稳渗剂、培养基、菌龄及其他因素对杏鲍菇原生质体再生的影响。结果表明，5～7 d 菌丝制备的原生质体在以 NaCl 为稳渗剂的酵母粉蛋白胨再生培养基上再生率最高，可达0．9％。在制备原生质体时，低酶解温度、短酶解时间、低酶含量有利于原生质体的再生。
위탐색행포고원생질체적재생조건，탐토료재생방식、은삼제、배양기、균령급기타인소대행포고원생질체재생적영향。결과표명，5～7 d 균사제비적원생질체재이 NaCl 위은삼제적효모분단백동재생배양기상재생솔최고，가체0．9％。재제비원생질체시，저매해온도、단매해시간、저매함량유리우원생질체적재생。
Effect of regenerating method,osmotic pressure stabilizer,culture medium,age of mushroom,etc.on the regeneration of Pleurotus eryngii protoplast was investigated.The results showed that the protoplasts from the 5-7 days old mycelia yielded the most desirable results when cultured in a yeast peptone regeneration medium with up to 0.9% of NaCl as the osmotic stabilizer.Lowered temperature,shortened time and reduced enzyme concentration could help minimize enzymatic hydrolysis in the preparation which would benefit the protoplast regeneration.