现代肿瘤医学
現代腫瘤醫學
현대종류의학
Journal of Modern Oncology
2015年
22期
3214-3217
,共4页
王磊%胡晨曦%夏铀铀%蒋晓东
王磊%鬍晨晞%夏鈾鈾%蔣曉東
왕뢰%호신희%하유유%장효동
胰腺癌%PANC -1%干细胞%ROS
胰腺癌%PANC -1%榦細胞%ROS
이선암%PANC -1%간세포%ROS
pancreatic adenocarcinoma%PANC -1%stem cell%ROS
目的：应用流式细胞仪从人胰腺癌细胞系 PANC －1中分选出肿瘤干性细胞，并对其生物学特性进行初步鉴定。方法：流式细胞仪分选出 CD44＋CD24＋、CD44－CD24＋、CD44＋CD24－和 CD44－CD24－4类细胞亚群；6MV X 射线照射前后，DCFH －DA 探针检查各亚群活性氧簇（reactive oxygen species，ROS）水平；将各亚群细胞接种于 BALB／C －nu／nu 裸小鼠，观察、比较成瘤率。结果：4个亚群细胞比例如下：CD44＋CD24＋（0．6±0．2）％、CD44＋CD24－（89．3±2．6）％、CD44－CD24＋（4．1±1．3）％和 CD44－CD24－（6．0±1．7）％；与其他亚群比较，CD44＋CD24＋干性细胞活性氧水平在 X 线照射后最低，平均荧光强度（MFI）显著低于其他3组细胞（P 值均＜0．01）。1×102个 CD44＋CD24＋细胞接种于裸鼠，6周后成瘤（1／8）；接种1×104个 CD44－CD24－细胞，12周后未成瘤。结论：分选出的各亚群中 CD44＋CD24＋更具有胰腺癌干细胞特性，体内成瘤能力最强，其余依次为 CD44＋CD24－、CD44－CD24＋、CD44－CD24－；CD44＋CD24＋细胞内 ROS 低水平，为进一步研究胰腺癌干性细胞 ROS 的基因调控奠定基础。
目的：應用流式細胞儀從人胰腺癌細胞繫 PANC －1中分選齣腫瘤榦性細胞，併對其生物學特性進行初步鑒定。方法：流式細胞儀分選齣 CD44＋CD24＋、CD44－CD24＋、CD44＋CD24－和 CD44－CD24－4類細胞亞群；6MV X 射線照射前後，DCFH －DA 探針檢查各亞群活性氧簇（reactive oxygen species，ROS）水平；將各亞群細胞接種于 BALB／C －nu／nu 裸小鼠，觀察、比較成瘤率。結果：4箇亞群細胞比例如下：CD44＋CD24＋（0．6±0．2）％、CD44＋CD24－（89．3±2．6）％、CD44－CD24＋（4．1±1．3）％和 CD44－CD24－（6．0±1．7）％；與其他亞群比較，CD44＋CD24＋榦性細胞活性氧水平在 X 線照射後最低，平均熒光彊度（MFI）顯著低于其他3組細胞（P 值均＜0．01）。1×102箇 CD44＋CD24＋細胞接種于裸鼠，6週後成瘤（1／8）；接種1×104箇 CD44－CD24－細胞，12週後未成瘤。結論：分選齣的各亞群中 CD44＋CD24＋更具有胰腺癌榦細胞特性，體內成瘤能力最彊，其餘依次為 CD44＋CD24－、CD44－CD24＋、CD44－CD24－；CD44＋CD24＋細胞內 ROS 低水平，為進一步研究胰腺癌榦性細胞 ROS 的基因調控奠定基礎。
목적：응용류식세포의종인이선암세포계 PANC －1중분선출종류간성세포，병대기생물학특성진행초보감정。방법：류식세포의분선출 CD44＋CD24＋、CD44－CD24＋、CD44＋CD24－화 CD44－CD24－4류세포아군；6MV X 사선조사전후，DCFH －DA 탐침검사각아군활성양족（reactive oxygen species，ROS）수평；장각아군세포접충우 BALB／C －nu／nu 라소서，관찰、비교성류솔。결과：4개아군세포비례여하：CD44＋CD24＋（0．6±0．2）％、CD44＋CD24－（89．3±2．6）％、CD44－CD24＋（4．1±1．3）％화 CD44－CD24－（6．0±1．7）％；여기타아군비교，CD44＋CD24＋간성세포활성양수평재 X 선조사후최저，평균형광강도（MFI）현저저우기타3조세포（P 치균＜0．01）。1×102개 CD44＋CD24＋세포접충우라서，6주후성류（1／8）；접충1×104개 CD44－CD24－세포，12주후미성류。결론：분선출적각아군중 CD44＋CD24＋경구유이선암간세포특성，체내성류능력최강，기여의차위 CD44＋CD24－、CD44－CD24＋、CD44－CD24－；CD44＋CD24＋세포내 ROS 저수평，위진일보연구이선암간성세포 ROS 적기인조공전정기출。
Objective:Flow cytometry was used to isolate tumor stem cells and research the culture and identifica-tion of tumor stem cells in pancreatic adenocarcinoma.Methods:Flow cytometry was used to isolate and sort the cells into 4 group according to the cell surface markers CD44 and CD24,CD44 +CD24 +,CD44 -CD24 +,CD44 +CD24 -, CD44 -CD24 -.ROS level was checked by DCFH -DA probe,the cells with different features were injected into BALB /C -nu/nu mice to determine the tumorrigenicity.Results:92.0% of sorted PANC -1 cells expressed the cell surface marker CD44,4.7% expressed CD24.The proportion of isolated cell:CD44 +CD24 +(0.6 ±0.2)%,CD44 +CD24 -(89.3 ±2.6)%,CD44 -CD24 +(4.1 ±1.3)%,CD44 -CD24 -(6.0 ±1.7)%.Comparing with the other groups,the ROS level of CD44 +CD24 + was lower (P <0.01).Implantation of 1 ×104 CD44 -CD24 -cells in nude mice,no tumor growth was ecident after 12 weeks.In contrast,nude mice impanted with 1 ×102 CD44 +CD24 + cells had tumor evident at 6 weeks(1 /8).Conclusion:The CD44 +CD24 +cells are highly tumorigenic subpopulation of pancreatic cancer cells and might be the stem cells of pancreatic adenocarcinomas.Tumor stem cells of pancreatic ad-enocarcinoma have properties of a lower level of ROS.
