CAJ | 학술논문

目的：检测和分选人类膀胱癌细胞系 T24中的侧群（side population，SP）细胞，并初步鉴定其癌干细胞特性，为癌干细胞的分离纯化奠定基础。方法：采用荧光激活细胞分选（FACS）技术，分选得到 T24细胞中的侧群细胞，并检测其比例。采用反转录聚合酶链反应（RT －PCR）技术检测侧群细胞中 ABCG2的表达情况；继而将侧群细胞培养于无血清培养基中，观察形成悬浮肿瘤细胞球的能力。结果：流式细胞分选结果显示：T24细胞中侧群细胞的比例约为3．6％。和对应的母系 T24细胞相比，T24侧群细胞的 ABCG2表达增高。培养于无血清培养基中的侧群细胞成簇生长，并形成肿瘤细胞球。结论：人类膀胱癌细胞系 T24中存在具有癌干细胞特性的侧群细胞。
목적：검측화분선인류방광암세포계 T24중적측군（side population，SP）세포，병초보감정기암간세포특성，위암간세포적분리순화전정기출。방법：채용형광격활세포분선（FACS）기술，분선득도 T24세포중적측군세포，병검측기비례。채용반전록취합매련반응（RT －PCR）기술검측측군세포중 ABCG2적표체정황；계이장측군세포배양우무혈청배양기중，관찰형성현부종류세포구적능력。결과：류식세포분선결과현시：T24세포중측군세포적비례약위3．6％。화대응적모계 T24세포상비，T24측군세포적 ABCG2표체증고。배양우무혈청배양기중적측군세포성족생장，병형성종류세포구。결론：인류방광암세포계 T24중존재구유암간세포특성적측군세포。
Objective:To identify the side population (SP)cells from human bladder cancer cell line T24 and characterize the cancer stem cell -like phenotype of these SP cells,which lays the foundation for the isolation and purification of cancer stem cells.Methods:Fluorescence activated cell sorting (FACS)was applied to isolate SP cells from T24 cell line.Reverse transcription -polymerase chain reaction (RT -PCR)was used to detect the expression of ABCG2 in T24 SP cells and parental T24 cells.The isolated SP cells were cultured in serum -free medium to ob-serve the capacity of growing into tumor spheres.Results:SP cells were isolated from T24 cells by flow cytometry,with a proportion of 3.6%.A higher expression of ABCG2 in SP cells was detected by RT -PCR in contrast to parental T24 cells.When cultured in serum -free medium,SP cells grew into sphere -like clusters/tumor spheres compared to the parental T24 cells.Conclusion:These multiple lines of evidence suggest that T24 cell line contained SP cells with cancer stem cell properties.