CAJ | 학술논문

目的：探讨应用 siRNA 技术沉默 S100A4基因表达后，对人膀胱癌细胞系 T －24增殖、凋亡及细胞侵袭能力的影响。方法：设计并合成 S100A4基因特异性的 siRNA 序列，转染人膀胱癌细胞系 T －24，48h 后应用 RT －PCR 和 Western blot 法检测在 mRNA 和蛋白水平 siRNA 对 S100A4的影响，MTT 法检测 T －24细胞增殖能力，流式细胞术检测转染 S100A4 siRNA 后 T －24细胞凋亡率，Transwell 法观察 siRNA 抑制 S100A4后对人膀胱癌细胞系侵袭能力的影响。结果：与空白对照组、阴性对照组相比，S100A4 siRNA 转染组 T －24细胞的 S100A4基因和蛋白表达降低（P ＜0．05）。MTT 法检测发现 S100A4 siRNA 转染组细胞增殖率明显下降（P＜0．01）；流式细胞术检测 siRNA 转染组细胞凋亡率高于其他各组，差异具有统计学意义（P ＜0．05）；Tran-swell 小室实验检测发现 T －24细胞侵袭能力明显下降（P ＜0．05）。结论：膀胱癌细胞中 S100A4表达与癌细胞侵袭、增殖和凋亡能力有关。S100A4 siRNA 能够抑制 T －24细胞 S100A4的表达，进而抑制细胞增殖和侵袭，促进细胞凋亡。S100A4基因和蛋白表达与膀胱癌的生物学特性密切相关，可能成为预测其发生转移和复发，以期指导临床治疗的重要指标。
목적：탐토응용 siRNA 기술침묵 S100A4기인표체후，대인방광암세포계 T －24증식、조망급세포침습능력적영향。방법：설계병합성 S100A4기인특이성적 siRNA 서렬，전염인방광암세포계 T －24，48h 후응용 RT －PCR 화 Western blot 법검측재 mRNA 화단백수평 siRNA 대 S100A4적영향，MTT 법검측 T －24세포증식능력，류식세포술검측전염 S100A4 siRNA 후 T －24세포조망솔，Transwell 법관찰 siRNA 억제 S100A4후대인방광암세포계침습능력적영향。결과：여공백대조조、음성대조조상비，S100A4 siRNA 전염조 T －24세포적 S100A4기인화단백표체강저（P ＜0．05）。MTT 법검측발현 S100A4 siRNA 전염조세포증식솔명현하강（P＜0．01）；류식세포술검측 siRNA 전염조세포조망솔고우기타각조，차이구유통계학의의（P ＜0．05）；Tran-swell 소실실험검측발현 T －24세포침습능력명현하강（P ＜0．05）。결론：방광암세포중 S100A4표체여암세포침습、증식화조망능력유관。S100A4 siRNA 능구억제 T －24세포 S100A4적표체，진이억제세포증식화침습，촉진세포조망。S100A4기인화단백표체여방광암적생물학특성밀절상관，가능성위예측기발생전이화복발，이기지도림상치료적중요지표。
Objective:To observe the effects of S100A4 gene silenced by siRNA on the proliferation,apoptosis and invasion ability of human bladder cell line T -24.Methods:The S100A4 siRNA was constructed and transfected into human bladder cell line T -24.After 48h,RT -PCR and Western blot were used to detect the inhibition effect of S100A4.MTT assay was performed to evaluate the effect of S100A4 gene silence on proliferation rate of T -24 cells. The apoptosis rates were detected by using flow cytometry.The invasion ability was detected by transwell method.Re-sults:Compared with blank transfected group and the negative control group,the expressions of S100A4 mRNA and protein were inhibited significantly in T -24 cells which was transfected with S100A siRNA(P <0.05).MTT assay found that proliferation rate of T -24 was obviously decreased in the transfected group(P <0.01).The apoptosis rate of siRNA transfection group was higher than other groups,respectively(P <0.05).Transwell results show that cell in-vasion ability was reduced in siRNA transfected group(P <0.05).Conclusion:S100A4 siRNA can significantly in-hibit the proliferation and invasion ability while enhanced the apoptosis of human bladder cell T -24.S100A4 may be a target of predicting the recurrence,metastasis and prognosis of bladder cancer,meanwhile provide guidance for clini-cal treatment.