CAJ | 학술논문

本研究采用 cDNA 末端快速扩增技术(RACE)结合 RT-PCR 方法克隆抗苄嘧磺隆雨久花生物型和敏感性雨久花生物型乙酰乳酸合成酶(ALS)基因 cDNA 序列，并对测序结果进行比对分析。结果表明：与敏感性的雨久花ALS 相比，公主岭(GZL)抗性生物型中第197位脯氨酸突变为组氨酸，第556位亮氨酸突变为苯丙氨酸；柳河(LH)抗性生物型中第358位天冬酰胺突变为天冬氨酸；磐石市(PS)抗性生物型中第525位缬氨酸突变为异亮氨酸。分析表明，高度保守区 Domain A 的第197位氨基酸残基的突变可能是导致公主岭稻区雨久花产生抗药性的主要原因之一，而其他抗性生物型抗性产生的原因有待进一步研究。
본연구채용 cDNA 말단쾌속확증기술(RACE)결합 RT-PCR 방법극륭항변밀광륭우구화생물형화민감성우구화생물형을선유산합성매(ALS)기인 cDNA 서렬，병대측서결과진행비대분석。결과표명：여민감성적우구화ALS 상비，공주령(GZL)항성생물형중제197위포안산돌변위조안산，제556위량안산돌변위분병안산；류하(LH)항성생물형중제358위천동선알돌변위천동안산；반석시(PS)항성생물형중제525위힐안산돌변위이량안산。분석표명，고도보수구 Domain A 적제197위안기산잔기적돌변가능시도치공주령도구우구화산생항약성적주요원인지일，이기타항성생물형항성산생적원인유대진일보연구。
Acetolactate synthetase(ALS )gene sequences were cloned from Monochoria korsakowii resistant (R) and susceptible (S)biotypes against bensulfuron-methyl using the rapid amplification of cDNA ends (RACE)and RT-PCR.Sequencing data revealed that Pro-1 97-His mutation and Leu-556-Phe mutation occurred in population GZL,while Asn-358-Asp mutation occurred in population LH,and Val-525-Ile mutation in population PS.The Pro-1 97-His mutation may be responsible for the resistance to bensulfuron-methyl in population GZL of M.korsa-kowii .The functions of the other three mutations in the R biotype need to be further investigated.