CAJ | 학술논문

目的：依据人大肠癌细胞 HCT －15的表面标志物，分离其中的干细胞亚群。建立裸鼠体内原代大肠癌荷瘤模型，比较各亚群肿瘤体积和质量。方法：利用免疫磁珠分选技术，分离其中 CD133／CD44干细胞亚群。分选得到 CD133＋CD44＋、CD133＋CD44－亚群分别接种于裸鼠体内，并观察肿瘤大小和质量。结果：CD133＋CD44＋和 CD133＋CD44－细胞亚群成功的从 HCT －15细胞中分离出来。接种 CD133＋CD44＋细胞的裸鼠形成的肿瘤体积[（2．76±0．22）cm3]和质量[（5．2±0．21）g]明显高于接种 CD133＋CD44－细胞裸鼠的肿瘤体积与质量[（1．56±0．34）cm3，（3．4±0．18）g]（P ＜0．05）。结论：CD44阳性的大肠癌肿瘤干细胞成瘤能力明显高于 CD44阴性的大肠癌肿瘤干细胞。
목적：의거인대장암세포 HCT －15적표면표지물，분리기중적간세포아군。건립라서체내원대대장암하류모형，비교각아군종류체적화질량。방법：이용면역자주분선기술，분리기중 CD133／CD44간세포아군。분선득도 CD133＋CD44＋、CD133＋CD44－아군분별접충우라서체내，병관찰종류대소화질량。결과：CD133＋CD44＋화 CD133＋CD44－세포아군성공적종 HCT －15세포중분리출래。접충 CD133＋CD44＋세포적라서형성적종류체적[（2．76±0．22）cm3]화질량[（5．2±0．21）g]명현고우접충 CD133＋CD44－세포라서적종류체적여질량[（1．56±0．34）cm3，（3．4±0．18）g]（P ＜0．05）。결론：CD44양성적대장암종류간세포성류능력명현고우 CD44음성적대장암종류간세포。
Objective:According to the surface markers of HCT -15 cancer cell,to isolate the stem cancer cells. To build colorectal cancer xenografts models and compare the tumor size and weight of SCID hairless congenic mice. Methods:We isolated the stem cancer cells from human colorectal cancer cell HCT -15 by magnetic microbeads. CD133 +CD44 + and CD133 +CD44 - stem cancer cells were subcutaneously inoculated to SCID hairless mice,then compared the tumor size and weight.Results:CD133 +CD44 + and CD133 +CD44 - cancer stem cells were isolated successfully from HCT -15 cells.The tumor size derived from CD133 +CD44 + cancer stem cells [(2.76 ±0.22) cm3 ]was larger than CD133 +CD44 - ones [(1.56 ±0.34)cm3 ](P <0.05).And the weight [(5.2 ±0.21)g]was also higher than negative ones [(3.4 ±0.18)g](P <0.05).Conclusion:The tumorigenicity of CD44 positive color-ectal stem cancer cells was significantly higher than negative ones.