CAJ | 학술논문

黄曲霉毒素污染严重影响着花生食品安全。通过常规育种的方式培育抗黄曲霉花生新品种进展缓慢，效果也难如人意。HIGS （Host －Induced Gene Silencing，寄主诱导的转基因沉默）技术是一种新兴的RNA 干扰技术，为培育抗病植物提供了可能。但该技术在花生上的应用尚未见报道。为创造抗黄曲霉花生新品系，本研究利用该技术构建了两个黄曲霉漆酶基因（Lac1和 Lac2）的 HIGS 载体，并试图将其转化到易感黄曲霉的花生品种粤油20中，获得了转 Lac1基因的 PCR 阳性种子。根据 HIGS 的原理，黄曲霉侵染后，转基因花生中产生的 dsRNA 将抑制黄曲霉漆酶基因表达，从而使转基因花生对黄曲霉产生抗性。基于该原理，下一步将对转基因种子是否抗黄曲霉侵染进行鉴定。本研究为利用 HIGS 技术探索黄曲霉漆酶基因与黄曲霉致病性的关系奠定了基础，为培育抗黄曲霉花生品种提供了一条新思路。
황곡매독소오염엄중영향착화생식품안전。통과상규육충적방식배육항황곡매화생신품충진전완만，효과야난여인의。HIGS （Host －Induced Gene Silencing，기주유도적전기인침묵）기술시일충신흥적RNA 간우기술，위배육항병식물제공료가능。단해기술재화생상적응용상미견보도。위창조항황곡매화생신품계，본연구이용해기술구건료량개황곡매칠매기인（Lac1화 Lac2）적 HIGS 재체，병시도장기전화도역감황곡매적화생품충월유20중，획득료전 Lac1기인적 PCR 양성충자。근거 HIGS 적원리，황곡매침염후，전기인화생중산생적 dsRNA 장억제황곡매칠매기인표체，종이사전기인화생대황곡매산생항성。기우해원리，하일보장대전기인충자시부항황곡매침염진행감정。본연구위이용 HIGS 기술탐색황곡매칠매기인여황곡매치병성적관계전정료기출，위배육항황곡매화생품충제공료일조신사로。
Aflatoxin contamination is a severe problem adversely affecting peanut food safety.Breeding a new peanut variety resistant to Aspergillus flavus is a lengthy process through conventional means,and the re-sults are far from satisfaction.Host -induced gene silencing (HIGS),as a novel technology for RNA interfer-ence,provides possibilities for creating disease -resistant crop plants.However,there is no reports on pea-nut.To breed new A.flavus -resistant peanut through genetic engineering,the HIGS vectors containing 2 lac-case genes,Lac1 and Lac2,were constructed and then were transformed into an A.flavus -susceptible peanut cultivar Yueyou 20.PCR -positive transgenic peanut seeds with Lac1 gene were obtained.According to the HIGS principle,if the transgenic peanut is infected by A.flavus,the laccase gene expression will be inhibited by dsRNA,thus the resistance of peanut to A.flavus will be induced.Based on this principle,whether the transgenic seeds had resistance to A.flavus infection would be identified next.The study layed foundations for exploring the relationship between laccase gene and A.flavus pathogenicity in peanut,and provided a new way for breeding peanut cultivars with A.flavus resistance.