黑龙江八一农垦大学学报
黑龍江八一農墾大學學報
흑룡강팔일농은대학학보
Journal of Heilongjiang August First Land Reclamation University
2015年
5期
111-115
,共5页
贾鹏禹%李朝阳%曹龙奎%李良玉%李洪飞%张丽萍
賈鵬禹%李朝暘%曹龍奎%李良玉%李洪飛%張麗萍
가붕우%리조양%조룡규%리량옥%리홍비%장려평
高效液相色谱%黄色素%小米%含量
高效液相色譜%黃色素%小米%含量
고효액상색보%황색소%소미%함량
HPLC%yellow pigment%millet%determination
小米中的黄色素是一类类胡萝卜素化合物。作为杂粮食品中的重要功能性成分,其成分和含量分析受到极大关注。建立了小米中黄色素物质的反相高效液相色谱分析方法。色谱分离柱采用YMCTM Carotenoid S-5(250×4.6 mm ID,5μm),流动相采用三元溶剂梯度洗脱模式,样品中目标组分采用甲基叔丁基醚(MTBE)超声波辅助萃取处理。色谱分离获得了较好的选择性,各黄色素组分17 min内完成基线分离,并在0.5~50.0μg ·mL-1浓度范围内呈现良好的线性关系,方法叶黄素、玉米黄素和β-隐黄素的检测限分别为0.3,0.4和0.4μg ·mL-1。该方法样品前处理简便,重现性好,结果准确。
小米中的黃色素是一類類鬍蘿蔔素化閤物。作為雜糧食品中的重要功能性成分,其成分和含量分析受到極大關註。建立瞭小米中黃色素物質的反相高效液相色譜分析方法。色譜分離柱採用YMCTM Carotenoid S-5(250×4.6 mm ID,5μm),流動相採用三元溶劑梯度洗脫模式,樣品中目標組分採用甲基叔丁基醚(MTBE)超聲波輔助萃取處理。色譜分離穫得瞭較好的選擇性,各黃色素組分17 min內完成基線分離,併在0.5~50.0μg ·mL-1濃度範圍內呈現良好的線性關繫,方法葉黃素、玉米黃素和β-隱黃素的檢測限分彆為0.3,0.4和0.4μg ·mL-1。該方法樣品前處理簡便,重現性好,結果準確。
소미중적황색소시일류류호라복소화합물。작위잡양식품중적중요공능성성분,기성분화함량분석수도겁대관주。건립료소미중황색소물질적반상고효액상색보분석방법。색보분리주채용YMCTM Carotenoid S-5(250×4.6 mm ID,5μm),류동상채용삼원용제제도세탈모식,양품중목표조분채용갑기숙정기미(MTBE)초성파보조췌취처리。색보분리획득료교호적선택성,각황색소조분17 min내완성기선분리,병재0.5~50.0μg ·mL-1농도범위내정현량호적선성관계,방법협황소、옥미황소화β-은황소적검측한분별위0.3,0.4화0.4μg ·mL-1。해방법양품전처리간편,중현성호,결과준학。
Yellow pigment in millet was a group of carotinoid compounds. As the important functional ingredients of cereal foodstuff, the component analysis of yellow pigment in millet was attracted extensive attention. In this paper,the determination method of yellow pigment in millet was established with reversed-phase high performance liquid chromatography. A YMC TM Carotenoid S-5 (250*4.6 mm ID,5 μm)column was used for the separation,and the mobile phase was subjected to gradient elution with triple-solvent. Samples were pretreated by ultrasonic wave assisted-extraction with methyl tert-butyl ether(MTBE).The separation obtained the highly sensitivity and selectivity. The separation was achieved with in 17 min. The calibration curve was linear in the mass concentration range of 0.5-50.0 μg·mL-1,and the LOD of lutein,zeaxanthin and β-cryptoxanthin were 0.3,0.4 and 0.4 μg·mL-1, respectively. The method was simple,accurate and reproducible.