植物保护
植物保護
식물보호
Plant Protection
2015年
5期
116-120
,共5页
赵玉强%田艳丽%罗金燕%姚红梅%余慧%陈磊%胡白石
趙玉彊%田豔麗%囉金燕%姚紅梅%餘慧%陳磊%鬍白石
조옥강%전염려%라금연%요홍매%여혜%진뢰%호백석
环介导等温扩增技术%瓜类细菌性果斑病菌%检测
環介導等溫擴增技術%瓜類細菌性果斑病菌%檢測
배개도등온확증기술%과류세균성과반병균%검측
loop-mediated isothermal amplification (LAMP)%Acidovorax citrulli%detection
本文利用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP),针对西瓜嗜酸菌(Aci-dovorax citrulli )基因组中 Aave_4063和 Aave_4064序列设计了2对特异引物 Ac-F3/Ac-B3和 Ac-FIP /Ac-BIP,建立了西瓜嗜酸菌的 LAMP 检测体系。利用该体系在65℃保温1 h,通过荧光显色即可完成检测。设计的引物特异性强,其检测灵敏度为2.0×101 cfu/mL。该方法为西瓜嗜酸菌的检疫及其所致病害的快速诊断提供了新的技术。
本文利用環介導等溫擴增技術(loop-mediated isothermal amplification,LAMP),針對西瓜嗜痠菌(Aci-dovorax citrulli )基因組中 Aave_4063和 Aave_4064序列設計瞭2對特異引物 Ac-F3/Ac-B3和 Ac-FIP /Ac-BIP,建立瞭西瓜嗜痠菌的 LAMP 檢測體繫。利用該體繫在65℃保溫1 h,通過熒光顯色即可完成檢測。設計的引物特異性彊,其檢測靈敏度為2.0×101 cfu/mL。該方法為西瓜嗜痠菌的檢疫及其所緻病害的快速診斷提供瞭新的技術。
본문이용배개도등온확증기술(loop-mediated isothermal amplification,LAMP),침대서과기산균(Aci-dovorax citrulli )기인조중 Aave_4063화 Aave_4064서렬설계료2대특이인물 Ac-F3/Ac-B3화 Ac-FIP /Ac-BIP,건립료서과기산균적 LAMP 검측체계。이용해체계재65℃보온1 h,통과형광현색즉가완성검측。설계적인물특이성강,기검측령민도위2.0×101 cfu/mL。해방법위서과기산균적검역급기소치병해적쾌속진단제공료신적기술。
The loop-mediated isothermal amplification (LAMP)was established for rapid detection of Acidovorax citrul-li .Four primers,Ac-F3/Ac-B3 and Ac-FIP/Ac-BIP,were designed specifically to recognize the Aave _4063 and Aave _4064 genes of A .citrulli .The LAMP products was obtained under isothermal conditions at 65 ℃ for 1 h.The result showed that no amplicon was observed except A .citrulli strains.The detection limits of pure cultures were 2.0×10 1 cfu/mL.This LAMP detection system provides a new alternative method for the rapid detection of A .citrulli .
