CAJ | 학술논문

为研究马链球菌马亚种新疆分离株 XZ1 SeM重组蛋白的免疫生物学功能,评价其作为抗原开展研制马链球菌疫苗的价值。采用PCR方法从马链球菌马亚种扩增其SeM基因,将其克隆至原核表达载体 pGEX-4T-2,诱导表达并用纯化的重组蛋白作免疫原免疫小鼠,分析比较重组蛋白免疫后小鼠抗体水平及对小鼠的免疫保护力。结果表明,纯化得到64 ku的 SeM重组蛋白,且该蛋白可与马链球菌马亚种阳性血清发生特异性反应。间接ELISA检测的抗体效价高达1∶128000,具有良好的免疫原性,免疫小鼠攻毒结果显示重组蛋白免疫对小鼠有良好的保护效果。
위연구마련구균마아충신강분리주 XZ1 SeM중조단백적면역생물학공능,평개기작위항원개전연제마련구균역묘적개치。채용PCR방법종마련구균마아충확증기SeM기인,장기극륭지원핵표체재체 pGEX-4T-2,유도표체병용순화적중조단백작면역원면역소서,분석비교중조단백면역후소서항체수평급대소서적면역보호력。결과표명,순화득도64 ku적 SeM중조단백,차해단백가여마련구균마아충양성혈청발생특이성반응。간접ELISA검측적항체효개고체1∶128000,구유량호적면역원성,면역소서공독결과현시중조단백면역대소서유량호적보호효과。
The immunological and biological activity of SeM recombinant protein of Streptococcus equi subspecies equi XZ1 Strain isolated in Xinjiang were studied in order to assess the value of SeM of S.equi for antigen development.The PCR method was applied to amplify SeM.The SeM gene was cloned into pGEX-4T-2 prokaryotic expression vector,and induced to express.The purified recombinant protein SeM were used to immunize mice.The antibody level for SeM immunization group and immunological protection of mice were analyzed after immunization.The result showed that there were 64 ku SeM recombinant pro-tein in the purification,and there could be pecullear reaction between the protein and the positive blood ser-um of S.equi.The antibody titer were 1∶128 000,which had good immunological effects.In addition,this recombinant protein had good protective effects for the mice after immunization.