植物保护
植物保護
식물보호
Plant Protection
2015年
5期
127-129,144
,共4页
耿肖兵%王春玲%黄铭慧%李永刚
耿肖兵%王春玲%黃銘慧%李永剛
경초병%왕춘령%황명혜%리영강
根腐病%大豆拟茎点种腐病菌%大豆%苗期%鉴定
根腐病%大豆擬莖點種腐病菌%大豆%苗期%鑒定
근부병%대두의경점충부병균%대두%묘기%감정
root rot%Phomopsis longicolla%Glycine max%seedling stage%identification
对10个引起大豆根腐病的真菌分离物进行鉴定。结果表明，这些分离物形态学上与大豆拟茎点种腐病菌相似。用 ITS 通用引物进行 PCR 扩增，该菌株与拟茎点种腐病菌 MP4PL11PS (GenBank 登录号 HQ130441.1)同源性达98%。因此，确定该菌株为大豆拟茎点种腐病菌。
對10箇引起大豆根腐病的真菌分離物進行鑒定。結果錶明，這些分離物形態學上與大豆擬莖點種腐病菌相似。用 ITS 通用引物進行 PCR 擴增，該菌株與擬莖點種腐病菌 MP4PL11PS (GenBank 登錄號 HQ130441.1)同源性達98%。因此，確定該菌株為大豆擬莖點種腐病菌。
대10개인기대두근부병적진균분리물진행감정。결과표명，저사분리물형태학상여대두의경점충부병균상사。용 ITS 통용인물진행 PCR 확증，해균주여의경점충부병균 MP4PL11PS (GenBank 등록호 HQ130441.1)동원성체98%。인차，학정해균주위대두의경점충부병균。
The 10 fungal isolates causing soybean root rot were identified.The results showed that 10 isolates had similarity to Phomopsis longicolla in morphology.BLAST search results showed that the sequence of P .longicolla strain MP4PL1 1PS (GenBank accession number HQ130441.1)was homologous to the tested strain with a similari-ty of 98% by using universal primers (ITS)for amplification.So,the isolated strain was identified as P .longicolla .
