新疆农业大学学报
新疆農業大學學報
신강농업대학학보
Journal of Xinjiang Agricultural University
2015年
4期
300-305
,共6页
贾莉莉%曲延英%王希东%姚正培%范李萍%王莉萍
賈莉莉%麯延英%王希東%姚正培%範李萍%王莉萍
가리리%곡연영%왕희동%요정배%범리평%왕리평
海岛棉%遗传多样性%SSR
海島棉%遺傳多樣性%SSR
해도면%유전다양성%SSR
sea-island cotton%genetic diversity%SSR
利用 SSR分子标记,选用34对带型清晰的多态性引物,对来自美国、前苏联、埃及和新疆自育的52份海岛棉种质资源进行遗传多样性分析.共检测到163个多态性位点,每组合的多态性条带数为2~7条,平均每个引物组合产生4.79条.利用 NTSYS-pc2.10e软件采用Jaccard’s相似系数,用 UPGMA法聚类分析绘制树状图.结果表明,大部分具有亲缘关系的品种聚在同类中,说明其结果与系谱具有一定的相符性,供试海岛棉品种之间的遗传相似系数为0.54~0.89,以遗传相似系数阈值为0.63时,可将52份海岛棉材料分成3大类:第一类包括新疆自育和来自美国品种,第二类以新疆自育品种为主,第三类包括前苏联和埃及品种.
利用 SSR分子標記,選用34對帶型清晰的多態性引物,對來自美國、前囌聯、埃及和新疆自育的52份海島棉種質資源進行遺傳多樣性分析.共檢測到163箇多態性位點,每組閤的多態性條帶數為2~7條,平均每箇引物組閤產生4.79條.利用 NTSYS-pc2.10e軟件採用Jaccard’s相似繫數,用 UPGMA法聚類分析繪製樹狀圖.結果錶明,大部分具有親緣關繫的品種聚在同類中,說明其結果與繫譜具有一定的相符性,供試海島棉品種之間的遺傳相似繫數為0.54~0.89,以遺傳相似繫數閾值為0.63時,可將52份海島棉材料分成3大類:第一類包括新疆自育和來自美國品種,第二類以新疆自育品種為主,第三類包括前囌聯和埃及品種.
이용 SSR분자표기,선용34대대형청석적다태성인물,대래자미국、전소련、애급화신강자육적52빈해도면충질자원진행유전다양성분석.공검측도163개다태성위점,매조합적다태성조대수위2~7조,평균매개인물조합산생4.79조.이용 NTSYS-pc2.10e연건채용Jaccard’s상사계수,용 UPGMA법취류분석회제수상도.결과표명,대부분구유친연관계적품충취재동류중,설명기결과여계보구유일정적상부성,공시해도면품충지간적유전상사계수위0.54~0.89,이유전상사계수역치위0.63시,가장52빈해도면재료분성3대류:제일류포괄신강자육화래자미국품충,제이류이신강자육품충위주,제삼류포괄전소련화애급품충.
Thirty four pairs of polymorphic primers from 52 sea-island cotton cultivars including USA, USSR,Egypt and Xinj iang were analyzed by using SSR markers.The total of 1 6 3 polymorphic loci were found,ther were polymorphic loci primers ranged from 2 to 7 with an average of 4.79.The NTSYS-pc2.10e software with Jaccard’s correlation coefficient were applied to analyze the dendrogram with UPGMA clus-tering dendrogram.The result showed that the most of the accessions with genetic relationships in their pedigrees were clustered into the same group,which showed that the result had a certain conform.The ge-netic resemble coefficient between the tested sea-island cotton cultivars was 0.54-0.89.When threshold value of genetic coefficient was 0.63,then 52 pairs of materials of sea-island cotton can be classified into three main groups:The first group was Xinjiang,the second group was Xinjiang,the third group was USSR and Egypt.