新疆农业大学学报
新疆農業大學學報
신강농업대학학보
Journal of Xinjiang Agricultural University
2015年
4期
296-299
,共4页
和田羊%KAP7%KAP8%多态性%羊毛长度
和田羊%KAP7%KAP8%多態性%羊毛長度
화전양%KAP7%KAP8%다태성%양모장도
Hetian sheep%KAP7%KAP8%polymorphism%the length of the wool
以采集的600只和田羊个体的血样为材料,利用 PCR-SSCP、DNA测序等技术研究分析和田羊KAP7、KAP8基因的多态性,并与羊毛长度进行关联.结果表明,和田羊KAP8基因没有发现多态性；KAP7基因具有多态性,存在3种基因型：AA、AB和BB,且处于 Hardy-Weinberg平衡状态(P＞0．05),多态信息含量为0．2376,属于低度多态.测序结果显示,与原基因碱基序列相比,KAP7基因碱基序列在110(T→G)和268(G→A)处发生突变.关联分析表明,和田羊不同基因型之间羊毛长度差异显著.
以採集的600隻和田羊箇體的血樣為材料,利用 PCR-SSCP、DNA測序等技術研究分析和田羊KAP7、KAP8基因的多態性,併與羊毛長度進行關聯.結果錶明,和田羊KAP8基因沒有髮現多態性；KAP7基因具有多態性,存在3種基因型：AA、AB和BB,且處于 Hardy-Weinberg平衡狀態(P＞0．05),多態信息含量為0．2376,屬于低度多態.測序結果顯示,與原基因堿基序列相比,KAP7基因堿基序列在110(T→G)和268(G→A)處髮生突變.關聯分析錶明,和田羊不同基因型之間羊毛長度差異顯著.
이채집적600지화전양개체적혈양위재료,이용 PCR-SSCP、DNA측서등기술연구분석화전양KAP7、KAP8기인적다태성,병여양모장도진행관련.결과표명,화전양KAP8기인몰유발현다태성；KAP7기인구유다태성,존재3충기인형：AA、AB화BB,차처우 Hardy-Weinberg평형상태(P＞0．05),다태신식함량위0．2376,속우저도다태.측서결과현시,여원기인감기서렬상비,KAP7기인감기서렬재110(T→G)화268(G→A)처발생돌변.관련분석표명,화전양불동기인형지간양모장도차이현저.
The blood samples of 600 Hetain sheep were applied as test materiats and PCR-SSCP,DNA se-quencing technology were used to research and analyze the genetic polymorphism of KAP7,KAP8 and carry out correlation with wool length.The result showed that there were no polymorphism in KAP 8 of Hetain sheep;However,there were polymorphism in KAP 7 gene of Hetain sheep,there were 3 genotypes:AA,AB and BB,and in the Hardy-Weinberg-equilibrium state (P>0.05),polymorphism information con-tent (PIC)was 0.2376,belonging to moderate polymorphism.Sequencing result showed that compared with the original genetic base sequence,KAP7 genetic base sequence was in 110G(T→G)and 268(G→A)point mutation.Correlation analysis showed that there were significant differences between different genotypes of Hetain sheep.
