CAJ | 학술논문

为研究马疱疹病毒1型gD 基因重组质粒与原核表达蛋白联合免疫效果,构建了真核表达载体 pVAX1-gD,将其转染至BHK-21细胞,经Western blot证实该基因可在BHK-21细胞中表达。将构建的gD 基因重组质粒与纯化后的gD蛋白质免疫小鼠,ELISA检测免疫小鼠血清抗体效价,用 MTT检测小鼠二免后的淋巴细胞增殖情况。结果表明,与对照组相比该联合免疫能有效的诱导免疫小鼠特异性抗体的产生,同时显著促进了特异性淋巴细胞的增殖。
위연구마포진병독1형gD 기인중조질립여원핵표체단백연합면역효과,구건료진핵표체재체 pVAX1-gD,장기전염지BHK-21세포,경Western blot증실해기인가재BHK-21세포중표체。장구건적gD 기인중조질립여순화후적gD단백질면역소서,ELISA검측면역소서혈청항체효개,용 MTT검측소서이면후적림파세포증식정황。결과표명,여대조조상비해연합면역능유효적유도면역소서특이성항체적산생,동시현저촉진료특이성림파세포적증식。
In order to study the immunological effect of combined immunization with the gD gene and its prokaryotic expressed protein,the eukaryotic expression vector was constructed and transfected BHK-21 cells,the expression of gD was confirmed by Western-Blot.The construction of gD recombinant plasmid and purified gD protein were used to immunize mice,the mice serum antibody titer was tested by ELISA, the mice lymphocyte proliferation was tested by MTT after second immunization.Our results indicatied that the combined immunization can effectively induce mice specific antibody and significantly promoted lymphocyte proliferation.