植物保护
植物保護
식물보호
Plant Protection
2015年
5期
174-178
,共5页
郑宽瑜%吴阔%董家红%方琦%张仲凯
鄭寬瑜%吳闊%董傢紅%方琦%張仲凱
정관유%오활%동가홍%방기%장중개
莴苣%番茄斑萎病毒%N 基因
萵苣%番茄斑萎病毒%N 基因
와거%번가반위병독%N 기인
lettuce%Tomato spotted wilt virus%N gene
莴苣类蔬菜是常见的食用蔬菜。近年在云南发现生菜、莴笋、油麦菜有症状表现为叶片黄化、褪绿、坏死斑的病害流行危害，为确定其病原，通过电子显微镜观察、回接试验、ELISA 检测、病毒基因分析，在典型症状病样组织粗汁液和超薄切片中均观察到有类似番茄斑萎病毒属病毒的粒体，该病毒粒体为球形，直径80~100 nm；ELISA 检测和病毒基因分析显示，侵染莴苣类蔬菜的病毒为番茄斑萎病毒。
萵苣類蔬菜是常見的食用蔬菜。近年在雲南髮現生菜、萵筍、油麥菜有癥狀錶現為葉片黃化、褪綠、壞死斑的病害流行危害，為確定其病原，通過電子顯微鏡觀察、迴接試驗、ELISA 檢測、病毒基因分析，在典型癥狀病樣組織粗汁液和超薄切片中均觀察到有類似番茄斑萎病毒屬病毒的粒體，該病毒粒體為毬形，直徑80~100 nm；ELISA 檢測和病毒基因分析顯示，侵染萵苣類蔬菜的病毒為番茄斑萎病毒。
와거류소채시상견적식용소채。근년재운남발현생채、와순、유맥채유증상표현위협편황화、퇴록、배사반적병해류행위해，위학정기병원，통과전자현미경관찰、회접시험、ELISA 검측、병독기인분석，재전형증상병양조직조즙액화초박절편중균관찰도유유사번가반위병독속병독적립체，해병독립체위구형，직경80~100 nm；ELISA 검측화병독기인분석현시，침염와거류소채적병독위번가반위병독。
Lettuce is an important dietary vegetable.In recent years,the virus disease causing yellowing,chlorosis, necrotic lesion symptoms on Lactuca sativa L.var.ramosa Hort.,Lactuca sativa L.var.longifolia Lam.,Lac-tuca sativa L.var.angustana Irish was prevalent in Yunnan Province.To determine the viral pathogen,electron microscopy,back-inoculation,ELISA,and virus gene analysis were used to detect the symptomatic samples.Typical tospovirus particles with a diameter of about 80~100 nm were observed in the sap of diseased tissues.ELISA,RT-PCR, and sequence analysis indicated that the virus present in the sampled lettuce plants was Tomato spotted wilt virus .
