温州医科大学学报
溫州醫科大學學報
온주의과대학학보
Journal of Wenzhou Medical University
2015年
10期
713-717,722
,共6页
张宇宁%杨训俊%沈飞霞%吕建新
張宇寧%楊訓俊%瀋飛霞%呂建新
장우저%양훈준%침비하%려건신
糖尿病,2型%线粒体DNA多态性%ND4%Meta分析
糖尿病,2型%線粒體DNA多態性%ND4%Meta分析
당뇨병,2형%선립체DNA다태성%ND4%Meta분석
diabetes mellitus,type 2%mitochondrial polymorphism%ND4%meta-analysis
目的：探讨线粒体DNANADH脱氢酶4亚基（ND4）基因12026A/G多态性与2型糖尿病（T2DM）的相关性。方法：通过对温州医科大学附属第一医院就诊的551例T2DM患者和757例对照者的线粒体DNA（mtDNA）进行PCR扩增以及Sanger测序，统计分析ND4基因12026A/G多态性与疾病的关系。采用logistic回归分析计算患病风险比（OR）和95%可信区间（CI）。同时通过搜索Pubmed、Embase、中国知网以及万方数据库，汇总已发表研究12026A/G多态性与T2DM关系的文献，提取数据，并利用Revman4.2和STATA10.0软件对数据进行Meta分析。结果：551例中国浙江籍T2DM患者中mtDNA12026G基因型占4.49%，757例非T2DM对照中mtDNA12026G基因型占4.75%，两组间差异无统计学意义（OR=0.94，95%CI：0.57～1.57；P=0.82）。然而汇总相关文献的Meta分析结果显示，MtDNA12026A/G多态性与T2DM的发生存在相关性（OR=1.95；95%CI：1.14～3.33，P=0.01）。结论：mtDNAND4基因12026G基因型是中国人群T2DM的风险因素，与T2DM的发生发展有关，但其在不同人群中存在差异。
目的：探討線粒體DNANADH脫氫酶4亞基（ND4）基因12026A/G多態性與2型糖尿病（T2DM）的相關性。方法：通過對溫州醫科大學附屬第一醫院就診的551例T2DM患者和757例對照者的線粒體DNA（mtDNA）進行PCR擴增以及Sanger測序，統計分析ND4基因12026A/G多態性與疾病的關繫。採用logistic迴歸分析計算患病風險比（OR）和95%可信區間（CI）。同時通過搜索Pubmed、Embase、中國知網以及萬方數據庫，彙總已髮錶研究12026A/G多態性與T2DM關繫的文獻，提取數據，併利用Revman4.2和STATA10.0軟件對數據進行Meta分析。結果：551例中國浙江籍T2DM患者中mtDNA12026G基因型佔4.49%，757例非T2DM對照中mtDNA12026G基因型佔4.75%，兩組間差異無統計學意義（OR=0.94，95%CI：0.57～1.57；P=0.82）。然而彙總相關文獻的Meta分析結果顯示，MtDNA12026A/G多態性與T2DM的髮生存在相關性（OR=1.95；95%CI：1.14～3.33，P=0.01）。結論：mtDNAND4基因12026G基因型是中國人群T2DM的風險因素，與T2DM的髮生髮展有關，但其在不同人群中存在差異。
목적：탐토선립체DNANADH탈경매4아기（ND4）기인12026A/G다태성여2형당뇨병（T2DM）적상관성。방법：통과대온주의과대학부속제일의원취진적551례T2DM환자화757례대조자적선립체DNA（mtDNA）진행PCR확증이급Sanger측서，통계분석ND4기인12026A/G다태성여질병적관계。채용logistic회귀분석계산환병풍험비（OR）화95%가신구간（CI）。동시통과수색Pubmed、Embase、중국지망이급만방수거고，회총이발표연구12026A/G다태성여T2DM관계적문헌，제취수거，병이용Revman4.2화STATA10.0연건대수거진행Meta분석。결과：551례중국절강적T2DM환자중mtDNA12026G기인형점4.49%，757례비T2DM대조중mtDNA12026G기인형점4.75%，량조간차이무통계학의의（OR=0.94，95%CI：0.57～1.57；P=0.82）。연이회총상관문헌적Meta분석결과현시，MtDNA12026A/G다태성여T2DM적발생존재상관성（OR=1.95；95%CI：1.14～3.33，P=0.01）。결론：mtDNAND4기인12026G기인형시중국인군T2DM적풍험인소，여T2DM적발생발전유관，단기재불동인군중존재차이。
Objective:To investigate the role of mitochondrial DNA 12026A/G (Mt12026) polymorphism in NADH-dehydrogenase subunit 4 (ND4) gene in T2DM. Methods:In order to detect 12026A/G polymorphism in ND4 gene, we used PCR and Sanger sequencing among 551 diabetic patients and 757 controls who were en-rolled from the First Afifliated Hospital of Wenzhou Medical University. Logistic regression analysis was used to estimate odds ratios (OR) and 95%conifdence intervals (CI). We searched through electronic database PubMed, Embase, China National Knowledge Infrastructure (CNKI) and Wanfang, a meta-analysis was employed based on the publications which described the association of the Mt12026A/G polymorphism with T2DM to evaluate the association between T2DM susceptibility and mitochondrial polymorphism in 12026A/G. Results:In our re-search, the genotype of mitochondrial DNA 12026G in 551 diabetic patients was 4.49%and 12026G genotype in 757 controls was 4.75%, no signiifcant association between diabetic patients and the controls for 12026A/G poly-morphism (OR=0.94, 95%CI 0.57~1.57, P=0.82) in Zhejiang province was found. But meta-analysis suggested that m.12026A/G polymorphism was associated with increased risk of T2DM (OR=1.95;95%CI 1.14~3.33, P=0.01). Conclusion:The Mt12026A/G polymorphism is associated with an increased risk of T2DM in Chinese Han population, but differences are existed among different populations.
