CAJ | 학술논문

为制备具有高效保护力的 C 型产气荚膜梭菌类毒素疫苗，利用 C 型产气荚膜梭菌标准菌株（NCTC 3180）所产外毒素经甲醛灭活并加入佐剂乳化后制备该疫苗。参照《中华人民共和国兽用生物制品质量标准》分别对疫苗进行了安全性检验、无菌检验和免疫效力检验。分别在分娩前30天和15天免疫接种怀孕母猪，通过间接 ELISA 和毒素中和试验评估母猪初乳和14日龄仔猪血液中抗体效价水平。结果显示，母猪初乳和仔猪血清中抗毒素效价分别为1∶3200和1∶1600，母猪初乳和仔猪血清中的中和效价均为1∶6。表明制备的类毒素疫苗效价高并安全可靠，免疫实验动物后能对母体和其所产幼畜起到一定的保护作用。
위제비구유고효보호력적 C 형산기협막사균류독소역묘，이용 C 형산기협막사균표준균주（NCTC 3180）소산외독소경갑철멸활병가입좌제유화후제비해역묘。삼조《중화인민공화국수용생물제품질량표준》분별대역묘진행료안전성검험、무균검험화면역효력검험。분별재분면전30천화15천면역접충부잉모저，통과간접 ELISA 화독소중화시험평고모저초유화14일령자저혈액중항체효개수평。결과현시，모저초유화자저혈청중항독소효개분별위1∶3200화1∶1600，모저초유화자저혈청중적중화효개균위1∶6。표명제비적류독소역묘효개고병안전가고，면역실험동물후능대모체화기소산유축기도일정적보호작용。
The exotoxin produced with Clostridium perfringens standard strain NCTC 31 80 was inactivated by 0.3% formalin and mixed with adjuvant to prepare the Clostridium perfringens type C toxoid vaccine.The safety,sterility and immune efficacy of the vaccine were tested according to the Veterinary Biological Products Quality Standards ofthe People’s Republic ofChina.The pregnant sows were vaccinated on the 30th and 1 5th day respectively before delivery,then the antitoxin titers of pregnant sow colostrum and 1 4 -day piglet serum were evaluated by indirect ELISA and toxin neutralization assays.The results showed that the antitoxin titers of colostrum and serum were 1 ∶3200 and 1 ∶1 600 respectively and their neutralization titers both were 1 ∶6.It proved that the prepared vaccine had high titer with safety and reliability and could protect the vaccinated ani-mals and their cubs.