CAJ | 학술논문

采用 Trizol 试剂法从榨菜叶中提取 RNA，采用 RT-PCR 技术扩增得到硫苷酶基因保守序列，利用 DNAman7生物信息分析软件对测得的保守序列与菜心硫苷酶基因保守序列进行比对，翻译核苷酸和蛋白质序列与芸薹属油菜、菜心、甘蓝、芥菜等序列进行相应的比对。结果表明，采用 Trizol 试剂法能获得320 bp 的基因保守序列；与甘蓝、菜心硫苷酶基因保守序列比对结果的 Identity 为97.12%；与芸薹属油菜、菜心、甘蓝等进行相应的翻译核苷酸和翻译成蛋白质序列比对分析，发现序列的覆盖度与相似度均达到较高的水平。
채용 Trizol 시제법종자채협중제취 RNA，채용 RT-PCR 기술확증득도류감매기인보수서렬，이용 DNAman7생물신식분석연건대측득적보수서렬여채심류감매기인보수서렬진행비대，번역핵감산화단백질서렬여예대속유채、채심、감람、개채등서렬진행상응적비대。결과표명，채용 Trizol 시제법능획득320 bp 적기인보수서렬；여감람、채심류감매기인보수서렬비대결과적 Identity 위97.12%；여예대속유채、채심、감람등진행상응적번역핵감산화번역성단백질서렬비대분석，발현서렬적복개도여상사도균체도교고적수평。
This purposes of this research is to lay the foundation for prokaryotic expression and to improve the content and activity of myrosinase in mustard brassica juncea at the same time. The research material was taken from myrosinase gene in mustard brassica juncea. This topic get conserved sequence of myrosinase gene taken from leaves for the method used, for extraction of RNA from mustard brassica juncea leaves that Trizol reagent step separation method of total RNA, according to the conserved nucleotide sequences of different plants and conservative sequence obtained by RT -PCR, conserved sequence alignment between the obtained and Brassica rape on the DNAman7 bioinformatics analysis software, myrosinase translated nucleotides sequence and protein sequence alignment analysis online combined the NCBI and main with Brassica napue, Brassica rape, Brassica oleracea, Brassica Juncea, etc. The results shows that under identical conditions: 320bp gene conserved sequence was obtained by method of Trizol reagent step separation, bioinformatics analysis suggested that conserved sequence showed 97.12% identity to M Y B. oleracea-M, M Y 49 B. parachinensis-M, myrosinase translated nucleotides sequence and protein sequence of Mustard brassica juncea has a higher query cover and identity with Brassica napue, Brassica rape, Brassica oleracea, etc. It found that the similarity of sequence coverage have reached a higher level.