CAJ | 학술논문

目的：通过比较第5天冷冻囊胚解冻培养4h与过夜培养20h后移植的临床结局，探讨囊胚解冻后到移植用以培养的适宜时间。方法：在第5天冷冻囊胚解冻移植周期中，按照囊胚解冻后到移植用以培养的时间不同分为2组：解冻4h后移植组（A组，移植周期157个，囊胚268枚）和提前解冻过夜培养20h后移植组（B组，移植周期227个，囊胚370枚），比较其妊娠结局。结果：B组的囊胚孵化率明显高于A组，差异有统计学意义（P＜0.01）；B组周期取消率稍高于A组，但差异无统计学意义（P＞0.05）；B组在平均移植胚胎个数上稍少于A组，但差异无统计学意义（P＞0.05）；A、B2组在临床妊娠率、胚胎种植率、流产率和多胎妊娠率上差异无统计学意义（P＞0.05）。结论：囊胚提前解冻过夜培养20h与培养4h后移植的临床结局无明显差异，操作人员对于囊胚解冻时间上将有更多选择。
목적：통과비교제5천냉동낭배해동배양4h여과야배양20h후이식적림상결국，탐토낭배해동후도이식용이배양적괄의시간。방법：재제5천냉동낭배해동이식주기중，안조낭배해동후도이식용이배양적시간불동분위2조：해동4h후이식조（A조，이식주기157개，낭배268매）화제전해동과야배양20h후이식조（B조，이식주기227개，낭배370매），비교기임신결국。결과：B조적낭배부화솔명현고우A조，차이유통계학의의（P＜0.01）；B조주기취소솔초고우A조，단차이무통계학의의（P＞0.05）；B조재평균이식배태개수상초소우A조，단차이무통계학의의（P＞0.05）；A、B2조재림상임신솔、배태충식솔、유산솔화다태임신솔상차이무통계학의의（P＞0.05）。결론：낭배제전해동과야배양20h여배양4h후이식적림상결국무명현차이，조작인원대우낭배해동시간상장유경다선택。
Objective:To compare the pregnancy outcomes when frozen blastocyst was transferred 4 hours after being thawed or 20 hours after being cultured overnight. Methods:For the 5 days freezing thawing blasto-cysts transplantation cycle, all the cycles are divided into two groups based on different culture time before trans-fer, those blastocysts transferred 4 hours after being thawed were labeled as A group (n=157) and those cultured overnight before being transferred the next day were included in B group (n=227). The pregnancy outcomes were compared between the two groups. Results:The proportion of thawing cycles leading to no transfer did not differ between groups (1.2%versus 2.2%for A and B, respectively). However, the proportion of hatched blastocysts de-rived from B group was signiifcantly higher than that from A group (41.7%versus 86.5%, respectively, P<0.001). All differences of the clinical pregnancy rate, implantation rate, miscarriage rate and multiple pregnancy rate between two groups have not yet reached the statistical signiifcance. Conclusion:Overnight culture of thawed blastocyst before transfer does not affect the main pregnancy outcomes. For blastocyst thawing time, operators will have more choices.