CAJ | 학술논문

目的：建立逆转录聚合酶链反应（RT －PCR）检测乳腺癌核内原癌基因（nuclear oncogene，MYC）和黏蛋白1（Mucin1，MUC1）基因表达水平，探讨其联合检测在乳腺癌诊断和治疗监测中的应用。方法：建立 RT －PCR 反应，并以β2－微球蛋白为内对照测定30例健康女性体检者、52例良性乳腺肿瘤和103例乳腺癌外周血中 MYC 和 MUC1的表达量。结果：外周血中 MYC 和 MUC1在乳腺癌组表达率显著高于良性乳腺疾病组及健康体检组，而良性乳腺疾病组和健康体检组的表达率无差异。MUC1和 MYC 联合检测的特异性显著高于单一基因。结论：RT －PCR 联合检测外周血 MUC1和 MYC 基因表达，可提高对乳腺癌诊断的特异性，具有标本来源容易、操作简便和价格便宜等优点，可有效辅助诊断乳腺癌。
목적：건립역전록취합매련반응（RT －PCR）검측유선암핵내원암기인（nuclear oncogene，MYC）화점단백1（Mucin1，MUC1）기인표체수평，탐토기연합검측재유선암진단화치료감측중적응용。방법：건립 RT －PCR 반응，병이β2－미구단백위내대조측정30례건강녀성체검자、52례량성유선종류화103례유선암외주혈중 MYC 화 MUC1적표체량。결과：외주혈중 MYC 화 MUC1재유선암조표체솔현저고우량성유선질병조급건강체검조，이량성유선질병조화건강체검조적표체솔무차이。MUC1화 MYC 연합검측적특이성현저고우단일기인。결론：RT －PCR 연합검측외주혈 MUC1화 MYC 기인표체，가제고대유선암진단적특이성，구유표본래원용역、조작간편화개격편의등우점，가유효보조진단유선암。
Objective:To evaluate combined detection of MYC and MUC1 gene in the diagnosis of breast cancer. Methods:We used RT -PCR to detect MYC and MUC1 mRNA express in clinical samples from 30 health women,52 patients with benign breast disease and 103 patients with breast cancer.β2 -microglobin was used as internal control. Results:No significant difference was found in the positive rate of MYC and MUC1 between normal controls and be-nign breast disease group(P >0.05).The positive rate of MYC and MUC1 in breast cancer group were higher than those in normal controls and benign breast disease group(P <0.05).The specificity of combined detection of MYC and MUC1 gene express was higher than that of single gene detection(P <0.05).Conclusion:Combined detection of MYC and MUC1 in peripheral blood by RT -PCR can improve the specificity of diagnosis of breast cancer,with spec-imens from easy,simple and cheap,which can effectively assist the diagnosis of breast cancer.