植物保护
植物保護
식물보호
Plant Protection
2015年
5期
207-211,224
,共6页
杜广祖%杨卫秋%周跃能%和淑琪%陈斌%桂富荣%李正跃
杜廣祖%楊衛鞦%週躍能%和淑琪%陳斌%桂富榮%李正躍
두엄조%양위추%주약능%화숙기%진빈%계부영%리정약
灰飞虱%黄绿绿僵菌%致死中时%致死中浓度
灰飛虱%黃綠綠僵菌%緻死中時%緻死中濃度
회비슬%황록록강균%치사중시%치사중농도
Laodelphax striatellus%Metarhizium flavoviride%LT50%LC50
为了筛选防治灰飞虱的高毒力生防真菌菌株及明确黄绿绿僵菌对灰飞虱的侵染致病温度条件,室内测定了黄绿绿僵菌 KM1104菌株在24~30℃下对灰飞虱成虫及3龄若虫的毒力。结果表明：黄绿绿僵菌菌株 KM1104对灰飞虱成虫和3龄若虫均具有较强的毒力,其中对成虫的毒力高于3龄若虫,在24~30℃下对成虫和3龄若虫的LC50分别为1.45×103~7.14×104和7.25×103~3.69×105孢子/mL,致死中时 LT50分别为3.02~11.53 d和6.85~11.58 d。不同温度下黄绿绿僵菌 KM1104菌株对灰飞虱的毒力不同,其中30℃下毒力最强,对成虫和3龄若虫的致死中浓度 LC50分别为1.45×103和7.25×103孢子/mL,接种1.01×105~1.01×108孢子/mL 后对成虫和3龄若虫的致死中时 LT50分别为3.02~6.86 d 和6.85~9.35 d。综合以上结果,黄绿绿僵菌菌株 KM1104具有良好的灰飞虱生防制剂开发潜力,且在30℃下使用致病力最强。
為瞭篩選防治灰飛虱的高毒力生防真菌菌株及明確黃綠綠僵菌對灰飛虱的侵染緻病溫度條件,室內測定瞭黃綠綠僵菌 KM1104菌株在24~30℃下對灰飛虱成蟲及3齡若蟲的毒力。結果錶明：黃綠綠僵菌菌株 KM1104對灰飛虱成蟲和3齡若蟲均具有較彊的毒力,其中對成蟲的毒力高于3齡若蟲,在24~30℃下對成蟲和3齡若蟲的LC50分彆為1.45×103~7.14×104和7.25×103~3.69×105孢子/mL,緻死中時 LT50分彆為3.02~11.53 d和6.85~11.58 d。不同溫度下黃綠綠僵菌 KM1104菌株對灰飛虱的毒力不同,其中30℃下毒力最彊,對成蟲和3齡若蟲的緻死中濃度 LC50分彆為1.45×103和7.25×103孢子/mL,接種1.01×105~1.01×108孢子/mL 後對成蟲和3齡若蟲的緻死中時 LT50分彆為3.02~6.86 d 和6.85~9.35 d。綜閤以上結果,黃綠綠僵菌菌株 KM1104具有良好的灰飛虱生防製劑開髮潛力,且在30℃下使用緻病力最彊。
위료사선방치회비슬적고독력생방진균균주급명학황록록강균대회비슬적침염치병온도조건,실내측정료황록록강균 KM1104균주재24~30℃하대회비슬성충급3령약충적독력。결과표명：황록록강균균주 KM1104대회비슬성충화3령약충균구유교강적독력,기중대성충적독력고우3령약충,재24~30℃하대성충화3령약충적LC50분별위1.45×103~7.14×104화7.25×103~3.69×105포자/mL,치사중시 LT50분별위3.02~11.53 d화6.85~11.58 d。불동온도하황록록강균 KM1104균주대회비슬적독력불동,기중30℃하독력최강,대성충화3령약충적치사중농도 LC50분별위1.45×103화7.25×103포자/mL,접충1.01×105~1.01×108포자/mL 후대성충화3령약충적치사중시 LT50분별위3.02~6.86 d 화6.85~9.35 d。종합이상결과,황록록강균균주 KM1104구유량호적회비슬생방제제개발잠력,차재30℃하사용치병력최강。
In order to screen the higher virulent entomopathogenic fungi and the optimal temperature for its infec-tion,the virulence of Metarhizium flavoviride strain KM1 104 against the adults and 3rd instar nymph of Laodel-phax striatellus was tested at different temperatures.The results showed that the strain KM1 104 was virulent against the adult and 3rd instar nymph of L .striatellus .The virulence against the adult was significantly higher than that against the 3rd instar nymph.The LC50 value for the adult and 3rd instar nymph was 1.45×10 3 -7.14× 10 4 and 7.25×10 3 -3.69×10 5 spores/mL,and the LT50 value was 3.02-1 1.53 d and 6.85-1 1.58 d,respective-ly.The virulence was significantly different at different temperature,the highest virulence was at temperature of 30 ℃,the LC50 value for the adult and 3rd instar nymph was 1.45×10 3 and 7.25 ×10 3 spores/mL,the LT50 value for the adult and 3rd instar nymph after inoculation of 1.01 × 10 5 - 1.01 × 10 8 spores/mL was 3.02 -6.86 d and 6.85- 9.35 d,respectively.Considering all the above,M.flavoviride strain KM1 104 had great potential for L .striatellus control,and the highest virulence was at 30 ℃.
