CAJ | 학술논문

文章为调查东北酸菜传统自然发酵过程中的真核微生物多样性,监测酸菜发酵体系生理生化动态变化,了解真核微生物在酸菜发酵过程中的作用.分析酸菜体系pH、可溶性糖、亚硝酸盐、乳酸、乙酸、乙醇和26S rDNA片段多样性.结果表明,发酵12 d时,酸菜体系pH从发酵初始值7.3下降到4.3后维持在4.1.可溶性糖发酵18 d时,由初始15.1%DM下降到4.5%DM.亚硝酸盐第6天时达到最大,随后下降.发酵体系中检测到的挥发性产物包括乳酸、乙酸和乙醇.发酵结束时,相应浓度分别达到6.8、0.78和32.2 g·L-1.26S rDNA D1/D2区变性梯度凝胶电泳结果显示发酵过程中真核微生物种类丰富.克隆文库揭示发酵第12天时,真核微生物主要为未培养的Stramenopile和土壤真菌,发酵30 d时,除上述两类微生物外还包括Candida sake,Cystofilobasidium infirmominia-tum,未培养Claclosporium和Tilletiopsis washingtonensis.土壤真菌(41%)、Candida sake(29%)、未培养Strameno-pile(17%)占所有检测真核微生物的86%.研究表明,Candida sake和Cystofilobasidium infirmominiatum对发酵体系中乙醇产生起一定作用.为研究酸菜发酵机制和控制酸菜发酵质量提供技术参考.
문장위조사동북산채전통자연발효과정중적진핵미생물다양성,감측산채발효체계생리생화동태변화,료해진핵미생물재산채발효과정중적작용.분석산채체계pH、가용성당、아초산염、유산、을산、을순화26S rDNA편단다양성.결과표명,발효12 d시,산채체계pH종발효초시치7.3하강도4.3후유지재4.1.가용성당발효18 d시,유초시15.1%DM하강도4.5%DM.아초산염제6천시체도최대,수후하강.발효체계중검측도적휘발성산물포괄유산、을산화을순.발효결속시,상응농도분별체도6.8、0.78화32.2 g·L-1.26S rDNA D1/D2구변성제도응효전영결과현시발효과정중진핵미생물충류봉부.극륭문고게시발효제12천시,진핵미생물주요위미배양적Stramenopile화토양진균,발효30 d시,제상술량류미생물외환포괄Candida sake,Cystofilobasidium infirmominia-tum,미배양Claclosporium화Tilletiopsis washingtonensis.토양진균(41%)、Candida sake(29%)、미배양Strameno-pile(17%)점소유검측진핵미생물적86%.연구표명,Candida sake화Cystofilobasidium infirmominiatum대발효체계중을순산생기일정작용.위연구산채발효궤제화공제산채발효질량제공기술삼고.
In this study, eukaryotic diversity during Northeast pickled cabbage fermentation was inves-tigated, and biochemical indices and microbial succession were measured. The pH decreased to 4.3 after 12 days of Northeast pickled cabbage fermentation from the initial 7.3, and then remained at approximately 4.1. Water-soluble carbohydrate content decreased from 15.1%to 4.5%of dry matter at 18 days of fermentation. At 6 days, nitrite content was the maximum, and then decreased dramatical y. Lactic acid, acetic acid and ethanol were the main volatile products identified. At the end of fermentation, the concentrations of lactic acid and acetic acid were 6.8 and 0.78 g·L-1, respectively. The ethanol concentration was 32.2 g·L-1. The results of 26S rDNA D1/D2 region DGGE and clone library analysis showed that eukaryotic diversity was rich during fermentation. The results from the cloning libraries revealed that the eukaryotic microorganisms detected in-cluded uncultured Stramenopile and uncultured soil fungus at 12 days fermentation. Candida sake, Cystofilo-basidium infirmominiatum,uncultured Claclosporium and Til etiopsis washingtonensis were also detected un-til 30 days of fermentation, in addition to the two species mentioned above. Uncultured soil fungus (41%), Candida sake (29%) and uncultured Stramenopile (17%) accounted for 86%of the eukaryotic microorgan-isms detected. The results indicated that Candida sake and Cystofilobasidium infirmominiatum (yeasts) con-tributed to ethanol production during the Northeast pickled cabbage fermentation. These results provided a comprehensive understanding of the traditional Northeast pickled cabbage fermentation process and a foun-dation for control ing Northeast pickled cabbage fermentation and quality.