东北农业大学学报
東北農業大學學報
동북농업대학학보
Journal of Northeast Agricultural University
2015年
9期
44-51
,共8页
孟祥晨%李雪%姚蕾%周金雨
孟祥晨%李雪%姚蕾%週金雨
맹상신%리설%요뢰%주금우
植物乳杆菌%溶血性%抗生素抗性%胃肠道耐受性%淋巴细胞增殖
植物乳桿菌%溶血性%抗生素抗性%胃腸道耐受性%淋巴細胞增殖
식물유간균%용혈성%항생소항성%위장도내수성%림파세포증식
Lactobacil us plantarum%hemolytic activity%antibiotic resistance%gastrointestinal toler-ance%lymphocyte proliferation
采用体外试验方法,评价两株植物乳杆菌KLDS1.0391与KLDS1.0706作为益生菌的潜在能力,包括对酸和胆盐的抗性、免疫活性以及安全性评价.采用活菌计数法测量其在低酸和胆盐条件下耐受性,采用MTT法观察不同剂量活性和热致死乳杆菌对小鼠脾脏淋巴细胞增殖能力影响,采用哥伦比亚血平板测定两株植物乳杆菌溶血性,采用K-B药敏纸片法测定其抗生素抗性.结果表明,两株植物乳杆菌均对酸和胆盐有较好耐受性;活性、热致死植物乳杆菌均能促进体外小鼠淋巴细胞增殖,表现出剂量依赖关系,活性植物乳杆菌作用效果较热致死乳杆菌更为明显;两株植物乳杆菌均无溶血性,对万古霉素与链霉素具有抗性.
採用體外試驗方法,評價兩株植物乳桿菌KLDS1.0391與KLDS1.0706作為益生菌的潛在能力,包括對痠和膽鹽的抗性、免疫活性以及安全性評價.採用活菌計數法測量其在低痠和膽鹽條件下耐受性,採用MTT法觀察不同劑量活性和熱緻死乳桿菌對小鼠脾髒淋巴細胞增殖能力影響,採用哥倫比亞血平闆測定兩株植物乳桿菌溶血性,採用K-B藥敏紙片法測定其抗生素抗性.結果錶明,兩株植物乳桿菌均對痠和膽鹽有較好耐受性;活性、熱緻死植物乳桿菌均能促進體外小鼠淋巴細胞增殖,錶現齣劑量依賴關繫,活性植物乳桿菌作用效果較熱緻死乳桿菌更為明顯;兩株植物乳桿菌均無溶血性,對萬古黴素與鏈黴素具有抗性.
채용체외시험방법,평개량주식물유간균KLDS1.0391여KLDS1.0706작위익생균적잠재능력,포괄대산화담염적항성、면역활성이급안전성평개.채용활균계수법측량기재저산화담염조건하내수성,채용MTT법관찰불동제량활성화열치사유간균대소서비장림파세포증식능력영향,채용가륜비아혈평판측정량주식물유간균용혈성,채용K-B약민지편법측정기항생소항성.결과표명,량주식물유간균균대산화담염유교호내수성;활성、열치사식물유간균균능촉진체외소서림파세포증식,표현출제량의뢰관계,활성식물유간균작용효과교열치사유간균경위명현;량주식물유간균균무용혈성,대만고매소여련매소구유항성.
The probiotic potential of two Lactobacil us plantarum strains KLDS1.0391, KLDS1.0706 was evaluated by a series of in vitro assays, and then a preliminary evaluation of the two strains of immuno-logical activity was conducted, final y the safety of two strains was evaluated. The tolerance to low pH and bile salt was used the viable counts assay;effect of active and thermal-inactive strains with different amount on the proliferative capacity of the lymphocytes in the mice spleen were assessed by the MTT method;in ad-dition, the hemolytic activity of two strains were determined by Columbia blood agar;the antibiotic resistance of two strains were determined by Kirby-Bauer (KB) antibiotic testing. Results showed that it was indicated that two non-hemolytic Lactobacil us plantarum strains were greatly resistant to harsh environment and had resistance to vancomycin and streptomycin. Though either the active Lactobacil us or the thermal-inactive Lactobacil us was able to promote the lymphocytes proliferation in vitro and the promoting effect was amount-dependent, active Lactobacil us were more effective than the thermal-inactive ones.