CAJ | 학술논문

采用盆栽人工控水方法,选择西北地区7份蚕豆主栽品种为材料,研究不同水分胁迫(轻度LS和重度WS)下蚕豆苗期地上部、地下部及叶片生理生化指标与抗旱性的关系.结果表明,干旱胁迫对蚕豆幼苗地上、地下部分均有不同程度影响,幼苗株高、茎粗、叶面积、侧根数量及蚕豆主根长度生长受到抑制,且随干旱胁迫程度加剧,抑制作用明显(P<0.05),临蚕9号株高、茎粗、叶面积、主根长度均表现显著抑制作用,为干旱敏感性蚕豆品种;超氧化物歧化酶(SOD)活性和叶绿素含量(Chl)在水分胁迫下总体呈先升后降趋势,青海13号SOD活性上升幅度较大,抗旱性较强.叶片可溶性糖含量(WSS)和细胞质膜相对透性(REC)随干旱胁迫加重均有明显增加,WSS增加越快,抗旱性越强,REC则相反,抗旱性越强的品种,细胞质膜透性增加越少,而蚕豆叶片组织相对含水率(RWC)比正常供水的下降明显,且下降幅度小的品种抗旱性越强;通过隶属函数法综合评价苗期抗旱性大小为:青海13号>临蚕6号>青蚕14号>青海12号>马牙>临蚕9号>临蚕8号;通过主成分分析和灰色关联分析,筛选出WSS、REC、RWC、SOD等生理指标和株高、叶面积、根长等形态指标,可作为蚕豆品种苗期抗旱能力评价必不可少的指标.
채용분재인공공수방법,선택서북지구7빈잠두주재품충위재료,연구불동수분협박(경도LS화중도WS)하잠두묘기지상부、지하부급협편생리생화지표여항한성적관계.결과표명,간한협박대잠두유묘지상、지하부분균유불동정도영향,유묘주고、경조、협면적、측근수량급잠두주근장도생장수도억제,차수간한협박정도가극,억제작용명현(P<0.05),림잠9호주고、경조、협면적、주근장도균표현현저억제작용,위간한민감성잠두품충;초양화물기화매(SOD)활성화협록소함량(Chl)재수분협박하총체정선승후강추세,청해13호SOD활성상승폭도교대,항한성교강.협편가용성당함량(WSS)화세포질막상대투성(REC)수간한협박가중균유명현증가,WSS증가월쾌,항한성월강,REC칙상반,항한성월강적품충,세포질막투성증가월소,이잠두협편조직상대함수솔(RWC)비정상공수적하강명현,차하강폭도소적품충항한성월강;통과대속함수법종합평개묘기항한성대소위:청해13호>림잠6호>청잠14호>청해12호>마아>림잠9호>림잠8호;통과주성분분석화회색관련분석,사선출WSS、REC、RWC、SOD등생리지표화주고、협면적、근장등형태지표,가작위잠두품충묘기항한능력평개필불가소적지표.
By the method of potted artificial water control ed, the article studied the relationship of aboveground, underground part and the leaf physiological and biochemical indices in seven northwest origin broad beans under drought stress (LS and WS). The results showed that drought stress had different influ-ence on aboveground, underground part of broad bean seedling, drought stress inhibited the growth of plant height, stem diameter, leaf area, taproot length and the number of lateral root of broad bean, with the in-crease of drought stress, inhibitory effect was obvious (P<0.05), there were significant differences on plant height, stem diameter, leaf area and taproot length of Lincan9, which was drought sensitive broad bean. With the increases of drought stress the activity of SOD and chlorophyl content showed an decreasing trend, Qinghai13 SOD activity had increased significantly, showed drought tolerance. By the increasing of drought stress, the leaf soluble sugar content and cel membrane permeability had increased significantly, the more quickly WSS decreased, the stronger drought tolerance broad bean had, on the contrary, if a broad bean had strong drought tolerance, the increase of REC was smal . While, the relative water content of broad bean leaf decreased more obviously than CK, and RWC of drought resistant cultivar decreased more slowly than the sensitive cultivar. The drought resistance of seven broad bean materials were arranged as the order from strong to weak: Qinghai13>Lincan6>Qingcan14>Qinghai12>Maya>Lincan9>Lincan8; Com-bined with principal components analysis and grey correlation analysis selected WSS, REC, RWC, SOD and plant height, leaf area, root length as broad bean seedling drought resistance evaluation indices.