CAJ | 학술논문

尿苷二磷酸葡萄糖脱氢酶(UGD)是多糖合成中的一个关键酶,广泛参与植物生长发育与非生物胁迫响应过程.采用生物信息学方法,在全基因组水平预测出10个大豆GmUGD基因;序列分析结果显示,GmUGD基因均具有UDP-葡萄糖/GDP-甘露糖脱氢酶家族的3个典型结构域;基因复制分析表明,80%GmUGD基因存在复制事件;系统发生分析结果显示,GmUGD基因分为三类,分类结果与基因复制分析结果基本吻合;基于UniGene数据库与GEO数据库的表达模式分析显示,根中GmUGD基因的ESTs最多,上胚轴中最少;另外,GmUGD基因能够响应盐碱胁迫诱导,在大豆根和叶中表达模式不同.结果可为进一步研究大豆GmUGD基因功能提供理论依据.
뇨감이린산포도당탈경매(UGD)시다당합성중적일개관건매,엄범삼여식물생장발육여비생물협박향응과정.채용생물신식학방법,재전기인조수평예측출10개대두GmUGD기인;서렬분석결과현시,GmUGD기인균구유UDP-포도당/GDP-감로당탈경매가족적3개전형결구역;기인복제분석표명,80%GmUGD기인존재복제사건;계통발생분석결과현시,GmUGD기인분위삼류,분류결과여기인복제분석결과기본문합;기우UniGene수거고여GEO수거고적표체모식분석현시,근중GmUGD기인적ESTs최다,상배축중최소;령외,GmUGD기인능구향응염감협박유도,재대두근화협중표체모식불동.결과가위진일보연구대두GmUGD기인공능제공이론의거.
UDP-glucose 6-dehydrogenase(UGD) is the key enzyme of polysaccharide biosynthesis, which plays important roles in the development and response to abiotic stresses of plant. A genome-wide analysis was conducted using bioinformatics and ten GmUGD genes of soybean were identified. Sequence analysis showed that al the GmUGD genes had three conserved domains which were typical in UDP-glucose/GDP-mannose dehydrogenase family. Gene duplication analysis indicated eighty percent of GmUGD genes were duplicated. Phylogenetic analysis showed that GmUGD genes were classified into three clusters, and the result was similar with gene duplication analysis. Expression pattern analysis which based on UniGene and GEO database indicated that more ESTs of GmUGDs were found in root than other tissues and none were found in epicotyls. In addition, the GmUGD genes responded to salinity-alkalinity stress and showed differential expression pattern under salinity-alkalinity stress. These results provide theoretical basis for further research of GmUGD genes function in soybean.