CAJ | 학술논문

目的：建立抑制性电导检测-离子色谱法快速测定水产品中常见的几种生物胺。方法以甲基磺酸(MSA)为淋洗液通过梯度洗脱分离目标生物胺,采用Ionpac CS17(4 mm×250 mm)分析柱和电导检测器进行分析。进样体积为25μL,采用峰面积定量。通过比较不同的提取剂(TCA(三氯乙酸)、HClO4、MSA)和提取方式(振荡、超声)的提取效果优化前处理方法。结果使用 MSA 超声提取效果较好,该方法测定生物胺的线性范围为0.02 mg~10.0 mg,检出限在0.5 mg/kg以下,加标回收率分别为85.2%~106.9%。结论该方法前处理简单快捷,结果灵敏、准确,适用于水产品中常见生物胺的快速检测。
목적：건립억제성전도검측-리자색보법쾌속측정수산품중상견적궤충생물알。방법이갑기광산(MSA)위림세액통과제도세탈분리목표생물알,채용Ionpac CS17(4 mm×250 mm)분석주화전도검측기진행분석。진양체적위25μL,채용봉면적정량。통과비교불동적제취제(TCA(삼록을산)、HClO4、MSA)화제취방식(진탕、초성)적제취효과우화전처리방법。결과사용 MSA 초성제취효과교호,해방법측정생물알적선성범위위0.02 mg~10.0 mg,검출한재0.5 mg/kg이하,가표회수솔분별위85.2%~106.9%。결론해방법전처리간단쾌첩,결과령민、준학,괄용우수산품중상견생물알적쾌속검측。
Objective To establish a method for the determination of common biogenic amines in seafood by ion chromatography method with suppressed conductance detection.Methods The samples were analyzed by Ionpac CS17(4 mm×250 mm) column and a conductance detector, with methylsulphonic acid (MSA) as the eluent to separate the amines in a gradient elution procedure. The injection loop was 25μL, and peak area was used for quantification. In this paper, different extracting agents (TCA (trichloroacetic acid), HClO4 and MSA) and methods (shaking and sonication) were applied for the biogenic amine extraction, and the outcomes were compared and optimized.ResultsThe sonication operation with MSA as extracting solution was proved to be the optimal pretreatment way. The linear range of the method was 0.02 mg~10.0 mg, the detection limit was below the 0.5 mg/kg, and the recovery of the amines ranged from 85.2%~106.9%. Conclusion The pretreatment of this method is simple and rapid, and the result is sensitive and precise, which makes it suitable for the application in biogenic amines determination in seafood.