CAJ | 학술논문

目的：利用不同前处理方法对乙酰丙酮法测定南美白对虾(Penaeus vannamei)中游离甲醛进行了研究,从而为水产品的中游离甲醛的提取、检测提供一定的理论基础和技术支持。方法通过对比高氯酸和三氯乙酸分别结合超声波和静置4种提取游离甲醛的前处理方法,对提取效果较好的前处理进行单因素和正交试验进行条件优化。结果高氯酸结合超声波提取游离甲醛的含量测定值最高,为1.68 mg/kg,回收率为79.53%,提取效果较好,对高氯酸浓度、超声波温度和超声波时间进行正交试验进行方差分析表明3个因素对游离甲醛提取效果的影响为：高氯酸浓度>超声波时间>超声波提取温度,高氯酸浓度影响极显著(P<0.01),最佳组合为：4%高氯酸作为提取剂,在30℃下超声35 min。结论本试验研究可作为提取南美白对虾中游离甲醛的较宜的前处理方法。
목적：이용불동전처리방법대을선병동법측정남미백대하(Penaeus vannamei)중유리갑철진행료연구,종이위수산품적중유리갑철적제취、검측제공일정적이론기출화기술지지。방법통과대비고록산화삼록을산분별결합초성파화정치4충제취유리갑철적전처리방법,대제취효과교호적전처리진행단인소화정교시험진행조건우화。결과고록산결합초성파제취유리갑철적함량측정치최고,위1.68 mg/kg,회수솔위79.53%,제취효과교호,대고록산농도、초성파온도화초성파시간진행정교시험진행방차분석표명3개인소대유리갑철제취효과적영향위：고록산농도>초성파시간>초성파제취온도,고록산농도영향겁현저(P<0.01),최가조합위：4%고록산작위제취제,재30℃하초성35 min。결론본시험연구가작위제취남미백대하중유리갑철적교의적전처리방법。
Objective To determine the content of 4 different preliminary treatments of extracting free formaldehyde inPenaeus vannameis by acetylacetone spectrophotometry, and provide reference for formaldehyde extracting and detecting in fishery products.Methods Trichloroacetic acid and perchloric acid combined with ultrasonic and soaking statically to extract free formaldehyde were compared respectively. And then, the single factor experiment and orthogonal experiment were carried out to improve the extracting effect. Results It showed that perchloric acid combined with ultrasound treatment reveal the highest content of free formaldehyde, which was 1.68 mg/kg, and the recovery rate was 79.53%. By analysis of variance, perchloric acid concentration had the biggest and significantly (P<0.01) influence on free formaldehyde extracting effect, and the influence of ultrasonictime was bigger than ultrasonic temperature. As a result,the most effective method for extracting free formaldehyde inPenaeus vannameihad been found out: 4% perchloric acid as extractant, and ultrasound treatment for 35 minutes at 30℃.Conclusion The reasearch can be used as appropriate pretreatment method for extracting free formaldehyde inPenaeus vannamei.