CAJ | 학술논문

目的：鲉从许氏平鱼皮中制备胶原蛋白及胶原肽，并对其特性进行初步探讨。方法通过氨基酸组成、傅里叶变换红外光谱(FTIR)、十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)及扫描电镜分析探讨鱼皮胶原蛋白的结构特征；制备不同分子量胶原肽组分SCH 1(Mw>2000 U)与SCH 2(Mw<2000 U)，测定其起泡性、吸水性及对 H2O2诱导 L929细胞氧化损伤的保护作用。结果氨基酸分析表明鱼皮胶原蛋白亚氨酸含量为16.7%； FTIR表明样品基本保持了天然三螺旋结构；扫描电镜分析显示冷冻干燥胶原蛋白具有多孔状结构。大分子量SCH 1的起泡性较好，而低分子量SCH 2具有较好的吸湿性。SCH对H2O2引起的细胞损伤产生一定的保护作用，并呈现出剂量效应关系，结论鲉许氏平鱼皮胶原蛋白为 I 型胶原蛋白，其胶原肽具有良好的起泡性与吸湿性，可有效抵御H2O2引起的细胞氧化损伤。
목적：유종허씨평어피중제비효원단백급효원태，병대기특성진행초보탐토。방법통과안기산조성、부리협변환홍외광보(FTIR)、십이완기광산납-취병희선알응효전영(SDS-PAGE)급소묘전경분석탐토어피효원단백적결구특정；제비불동분자량효원태조분SCH 1(Mw>2000 U)여SCH 2(Mw<2000 U)，측정기기포성、흡수성급대 H2O2유도 L929세포양화손상적보호작용。결과안기산분석표명어피효원단백아안산함량위16.7%； FTIR표명양품기본보지료천연삼라선결구；소묘전경분석현시냉동간조효원단백구유다공상결구。대분자량SCH 1적기포성교호，이저분자량SCH 2구유교호적흡습성。SCH대H2O2인기적세포손상산생일정적보호작용，병정현출제량효응관계，결론유허씨평어피효원단백위 I 형효원단백，기효원태구유량호적기포성여흡습성，가유효저어H2O2인기적세포양화손상。
ObjectiveThe collagen and collagen peptides were prepared from rockfish skin and the characterization was investigated.MethodsThe properties of collagen were analyzed by amino acid analysis, Fourier transform infrared spectroscopy(FTIR), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscope (SEM). SCH 1 (Mw>2000 U) and SCH 2 (Mw<2000 U) were prepared using ultrafiltration membranes. The water absorption capacity, moisture retention capacity, foaming expansion, foaming stability, and antioxidant activity in H2O2-stimulated L929 cells were analyzed. ResultsThe amino acid composition showed the amino acid content of collagen was 16.7%; FTIR spectra showed collagen remained natural triple-helix structure. Collagen displayed a polyporous ultrastructure under SEM observation. SCH 1 possessed better foaming properties, SCH 2 showed a better water absorption capacity. SCH exhibited certain protective effect against cell damage induced by H2O2.ConclusionThe collagen extracted from the rockfish skin was type I collagen. SCH had good foaming properties and water absorption capabilities. Treatment of H2O2-induced L929 cells with SCH increased cell viability.