CAJ | 학술논문

目的：了解长牡蛎(Crassostrea gigas)消化道组织中类组织血型抗原(histo-blood group antigens, HBGAs)的类型特点,分析其与诺如病毒的结合特性,以探讨长牡蛎富集诺如病毒的机制。方法利用8种HBGAs单克隆抗体,建立长牡蛎中类HBGAs检测的ELISA方法,并分析其主要型别。同时,利用5种体外表达的 GII.4型诺如病毒 P 粒子分析其与长牡蛎中类 HBGA 的结合特性。结果长牡蛎消化道组织中存在类A, H1, Lea和Ley型HBGA；55019株(2006b变异株)和97-1l株(95/96US变异株) GII.4型诺如病毒P粒子可通过类A、H1和Ley型HBGA与长牡蛎消化道组织相结合,91(Camberwell_91株)和42(Hunter_2004)株可通过类Ley型HBGA与长牡蛎消化道组织相结合,156株(sakai株)不与任何类型类HBGA结合。结论长牡蛎消化道组织中存在类A、H1、Lea和Ley型HBGA, GII.4型诺如病毒主要通过类A、H1和Ley型HBGA与长牡蛎消化道组织相结合。
목적：료해장모려(Crassostrea gigas)소화도조직중류조직혈형항원(histo-blood group antigens, HBGAs)적류형특점,분석기여낙여병독적결합특성,이탐토장모려부집낙여병독적궤제。방법이용8충HBGAs단극륭항체,건립장모려중류HBGAs검측적ELISA방법,병분석기주요형별。동시,이용5충체외표체적 GII.4형낙여병독 P 입자분석기여장모려중류 HBGA 적결합특성。결과장모려소화도조직중존재류A, H1, Lea화Ley형HBGA；55019주(2006b변이주)화97-1l주(95/96US변이주) GII.4형낙여병독P입자가통과류A、H1화Ley형HBGA여장모려소화도조직상결합,91(Camberwell_91주)화42(Hunter_2004)주가통과류Ley형HBGA여장모려소화도조직상결합,156주(sakai주)불여임하류형류HBGA결합。결론장모려소화도조직중존재류A、H1、Lea화Ley형HBGA, GII.4형낙여병독주요통과류A、H1화Ley형HBGA여장모려소화도조직상결합。
Objective To know the features of type-like HBGAs in digestive tissue ofCrassostrea gigas, and analyze its binding properties of GII.4 genotypenorovirus variants, and to research the mechanism of enrichment.MethodsEight types HBGAs-specific monoclonal antibodies (MAbs) from human were used to establish the ELISA method for type-like HBGAs inCrassostrea gigas. Binding profile between type-like HBGAs inCrassostrea gigas and 5 GII.4 genotypenorovirus variants were also analyzed.ResultsA-like, H1-like, Lea-like and Ley-like were the main types HBGAs existed in digestive tissue ofCrassostrea gigas. 2006b variant and 95/96US variant binding with A-like, H1-like and ley-like HBGAs inCrassostrea gigas. However, Camberwell_91 variant and Hunter_2004 variant only bind with ley-like HBGA, and sakai variant bind no type HBGA inCrassostrea gigas.Conclusion There are A-like, H1-like, Lea-like and Ley-like type HBGAs in digestive tissue ofCrassostrea gigas. GII.4 genotypenorovirus variants mainly bind with A-like , H1-like and Ley-like HBGA inCrassostrea gigas.