CAJ | 학술논문

目的：以廉价菜粕作为氮源,对其直接生物利用过程中的碳源种类和培养模式进行优化和改进。方法研究液态静置培养模式下细胞生长和伊枯草菌素生产变化特性,在此基础上提出一种两阶段(振荡+静态)组合式培养模式。结果麸皮作为碳源最有利于伊枯草菌素表达,最高浓度是葡萄糖作为碳源时最高产量的1.6倍；液态静置培养基表面能够形成厚而稳定的生物膜,发酵中后期具有比振荡培养更高的伊枯草菌素生产强度；采用液态振荡和静置组合培养方式伊枯草菌素最高浓度可达1.10 g/L,接近完全振荡培养时的最高水平(1.16 g/L)。结论相对于传统的全程式振荡培养而言,这种新的组合培养方式不仅有利于伊枯草菌素高产期(发酵中后期)的过程控制,还能降低整个发酵过程的动力成本。
목적：이렴개채박작위담원,대기직접생물이용과정중적탄원충류화배양모식진행우화화개진。방법연구액태정치배양모식하세포생장화이고초균소생산변화특성,재차기출상제출일충량계단(진탕+정태)조합식배양모식。결과부피작위탄원최유리우이고초균소표체,최고농도시포도당작위탄원시최고산량적1.6배；액태정치배양기표면능구형성후이은정적생물막,발효중후기구유비진탕배양경고적이고초균소생산강도；채용액태진탕화정치조합배양방식이고초균소최고농도가체1.10 g/L,접근완전진탕배양시적최고수평(1.16 g/L)。결론상대우전통적전정식진탕배양이언,저충신적조합배양방식불부유리우이고초균소고산기(발효중후기)적과정공제,환능강저정개발효과정적동력성본。
Objective To optimize and improve the type of carbon source and culture model in the process of bio-utilization when using rapeseed meal as a nitrogen source.MethodsA combined culture mode of first shaking culture following by static biofilm fermentation was proposed based on the changing characteristics ofBacillus subtilis growth and iturin A production under static culture.Results It was indicated that wheat bran was the best carbon source for iturin A production, and the maximum iturin A concentration was 1.6 times higher than that with glucose as a carbon source. Thick and stable biofilm was observed when adopting static culture, and the iturin A productivity was higher than that with traditional shaking culture during the later period of fermentation. Compared to single static culture, the proposed combined culture mode could further improve iturin A production, and the maximum iturin A concentration reached 1.10 g/L, which was close to the highest level produced with single shaking culture (1.16 g/L).Conclusion This new culture strategy can not only decrease the power cost but also can benefit to process control of iturin A in the later period of fermentation.