CAJ | 학술논문

目的：比较桦褐孔菌醇提物不同极性部位的抗氧化活性,从中筛选出抗氧化的功能部位。方法采用系统溶剂法分离桦褐孔菌菌核的乙醇提取物,分别得到石油醚相、氯仿相、乙酸乙酯相和乙醇相等4个不同极性部位,以还原力及对2,2-二(4-叔辛基苯基)-1-苦肼基自由基(DPPH·)和羟自由基(·OH)的清除能力作为筛选其抗氧化活性的评价指标,并对4种萃取物所含有的黄酮含量和多酚含量进行测定。结果4种提取物均具有较高的抗氧化活性,还原力及清除 DPPH·和·OH 的活性大小顺序均为：乙醇相>乙酸乙酯相>氯仿相>石油醚相,并与多酚含量和黄酮含量具有很高的相关性。乙醇相的清除率最高,在500μg/mL时,对DPPH·和·OH的清除率分别高达83.13%和52.44%,非常接近于阳性对照抗坏血酸的清除活性。结论乙醇相是主要的抗氧化活性部位,值得进一步开发利用。
목적：비교화갈공균순제물불동겁성부위적항양화활성,종중사선출항양화적공능부위。방법채용계통용제법분리화갈공균균핵적을순제취물,분별득도석유미상、록방상、을산을지상화을순상등4개불동겁성부위,이환원력급대2,2-이(4-숙신기분기)-1-고정기자유기(DPPH·)화간자유기(·OH)적청제능력작위사선기항양화활성적평개지표,병대4충췌취물소함유적황동함량화다분함량진행측정。결과4충제취물균구유교고적항양화활성,환원력급청제 DPPH·화·OH 적활성대소순서균위：을순상>을산을지상>록방상>석유미상,병여다분함량화황동함량구유흔고적상관성。을순상적청제솔최고,재500μg/mL시,대DPPH·화·OH적청제솔분별고체83.13%화52.44%,비상접근우양성대조항배혈산적청제활성。결론을순상시주요적항양화활성부위,치득진일보개발이용。
Objective To compare the biological activities of different polar parts with each other, in order to screen functional parts of ethanol extract fromInonotus obliquus sclerotia.Methods The systematic solvent extraction was used to extract and separate the ethanol extract by petroleum ether (PE), chloroform (Chl), ethyl acetate (EA) and ethanol (Eth), respectively. Accordingly, 4 extracts were obtained and named PEE, ChlE, EAE and EthE, respectively. Their antioxidant capacitiesin vitro were investigated in terms of reducing power assay and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and hydroxyl radicals. Furthermore, the flavonoid content and polyphenol content of 4 extracts were determined.ResultsIn general, 4 kinds of extracts exhibited increasing antioxidant activity with increasing concentration at the ranges of tested dosage. The orders of reducing power, DPPH-scavenging capacity and hydroxyl-scavenging activity were all EthE>EAE>PEE>ChlE, which was very relevant to the polyphenol content and flavonoids content. These findings demonstrated that 4 extracts extracted fromI. obliquus, especially EthE, at 500 μg/mL, the scavenging rate on DPPH and OH radicals were 83.13% and 52.44%, respectively, which was near to that of the ascorbic acid that was served as positive control.Conclusion The EthE can be employed as natural ingredients in functional food and pharmaceutical industry to alleviate the oxidative stress.