CAJ | 학술논문

目的：充分利用红枣多糖资源，发掘碱提红枣多糖生物活性。方法以热水浸提红枣多糖剩余的残渣为原料，采用氢氧化钠溶液进一步提取其中的多糖物质，获得碱提红枣多糖，对其基本理化性质和生物活性进行研究，并与热水浸提红枣多糖进行比较。结果碱提红枣多糖对光照核黄素产生的超氧阴离子自由基和DPPH 自由基具有较强的清除作用，并可强力抑制α-葡萄糖苷酶和透明质酸酶活性。与水提红枣多糖相比，碱提红枣多糖在低浓度时对超氧阴离子自由基的清除作用显著提高，对α-葡萄糖苷酶活性的半抑制剂量降低99%以上。同浓度条件下，碱提红枣多糖对 DPPH 的清除率明显高于水提红枣多糖，而且随着浓度的提高，其差别更加显著。碱提红枣多糖对透明质酸酶的抑制作用在低浓度条件下显著弱于水提红枣多糖，但当浓度提高到1.0 mg/mL以上时，碱提红枣多糖对透明质酸酶活性的抑制率超过水提红枣多糖。结论碱提红枣多糖是一种优良的自由基清除剂和α-葡萄糖苷酶及透明质酸酶抑制剂，而且部分活性优于水提红枣多糖，利用碱提工艺可提高红枣资源的利用率，又获得了高活性的红枣多糖。
목적：충분이용홍조다당자원，발굴감제홍조다당생물활성。방법이열수침제홍조다당잉여적잔사위원료，채용경양화납용액진일보제취기중적다당물질，획득감제홍조다당，대기기본이화성질화생물활성진행연구，병여열수침제홍조다당진행비교。결과감제홍조다당대광조핵황소산생적초양음리자자유기화DPPH 자유기구유교강적청제작용，병가강력억제α-포도당감매화투명질산매활성。여수제홍조다당상비，감제홍조다당재저농도시대초양음리자자유기적청제작용현저제고，대α-포도당감매활성적반억제제량강저99%이상。동농도조건하，감제홍조다당대 DPPH 적청제솔명현고우수제홍조다당，이차수착농도적제고，기차별경가현저。감제홍조다당대투명질산매적억제작용재저농도조건하현저약우수제홍조다당，단당농도제고도1.0 mg/mL이상시，감제홍조다당대투명질산매활성적억제솔초과수제홍조다당。결론감제홍조다당시일충우량적자유기청제제화α-포도당감매급투명질산매억제제，이차부분활성우우수제홍조다당，이용감제공예가제고홍조자원적이용솔，우획득료고활성적홍조다당。
Objective To utilize jujube polysaccharide resources fully and discover bioactivities of polysaccharides fromZizyphus jujuba fruit extracted with alkaline solution (AZJP).Methods AZJP was extracted from remainder residues of hot water extraction ofZizyphus jujuba fruit by using 0.1 mol/L of NaOH as solvent. Then the physico-chemical characteristics and bioactivities of AZJP were studied as compared with Zizyphus jujuba fruit polysaccharide extracted with hot water (WZJP).Results AZJP had strong scavenging effects on the superoxide radicals generating from illuminating riboflavin as well as DPPH radicals, and could inhibit the activities ofα-glucosidase and hyaluronidase potently. As compared with WZJP, AZJP exhibited a stronger scavenging effect towards superoxide radicals at low concentration, and the IC50 forα-glucosidase lowered 99%. The DPPH scavenging percentage of AZJP was significantly higher than that of WZJP at the same concentration, and the differences became more significant with the increasing of sample concentration.As for hyaluronidase inhibitory activity, the inhibition percentage of AZJP was significantly lower than that of WZJP at low concentration, but it became higher than that of WZJP when the sample concentration was increased to above 1.0 mg/mL.ConclusionAZJP is a good free radical scavenger,α-glucosidase and hyaluronidase inhibitor, which is partly superior to WZJP. These results suggest that the AZJP may play an important role in human health and disease prevention.By using alkaline extraction technique, the utilization ofZizyphus jujuba fruit resources is improved and some new polysaccharides with high bioactivities may be obtained.