CAJ | 학술논문

目的：建立豆芽中4-氯苯氧乙酸钠的液相色谱-串联质谱测定方法。方法豆芽样品均质后以0.01 mol/L氢氧化钠溶液提取,提取液经酸化后再用乙酸乙酯萃取,收集经离心分层的有机相,浓缩后用甲醇溶解进行测定分析。以甲醇和0.1%甲酸(FA)水溶液为流动相,采用Hypersil GOLD aQ (100 mm×2.1 mm,3μm)色谱柱分离,电喷雾电离负离子条件下以选择反应监测模式(SRM)检测。结果该方法检出限0.005 mg/kg,在1~100 ng/mL范围内具有良好线性,相关系数为0.9998。回收率为79.1%~104.7%,相对标准偏差低于5%(n=6)。结论该方法前处理简单快速,定性准确,灵敏度好,适用于豆芽中4-氯苯氧乙酸钠的残留检测。
목적：건립두아중4-록분양을산납적액상색보-천련질보측정방법。방법두아양품균질후이0.01 mol/L경양화납용액제취,제취액경산화후재용을산을지췌취,수집경리심분층적유궤상,농축후용갑순용해진행측정분석。이갑순화0.1%갑산(FA)수용액위류동상,채용Hypersil GOLD aQ (100 mm×2.1 mm,3μm)색보주분리,전분무전리부리자조건하이선택반응감측모식(SRM)검측。결과해방법검출한0.005 mg/kg,재1~100 ng/mL범위내구유량호선성,상관계수위0.9998。회수솔위79.1%~104.7%,상대표준편차저우5%(n=6)。결론해방법전처리간단쾌속,정성준학,령민도호,괄용우두아중4-록분양을산납적잔류검측。
Objective To establish a method for determination of sodium parachlorophenoxy (4-CPANa) in bean sprouts by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). MethodsThe soybean sample was extracted with 0.01 mol/L sodium hydroxide solution, and the extracting solution was recovered by diethyl ether in acidic condition. After centrifugation, the organic phase was concentrated completely and re-dissolved with methanol. The sample was separated by a Hypersil GOLD aQ column (100 mm×2.1 mm, 3μm) with methanol and 0.1% FA aqueous as mobile phase. The remnant was detected by MS/MS using H-ESI technology (negative ion mode) and SRM detection mode.ResultsThe detection limit was 0.005 mg/kg and the linear range was 1~100 ng/mL with correlation coefficient (r2) of 0.9998. The average recoveries were between 79.1%~104.7% with relative standard deviation (RSD) less than 5% (n=6).ConclusionThisanalytical method is sample, rapid, accurate, and sensitive, and can be used for the rapid detection of sodium parachlorophenoxy in bean sprouts.