CAJ | 학술논문

目的：建立了液相色谱-串联质谱测定婴幼儿食品中6种黄曲霉毒素的方法。方法试样经乙腈超声提取,离心, MycosepTM226净化柱净化后液相色谱串联质谱仪直接测定,外标法定量。结果加标回收率范围为83.3%~100.6%,相对标准偏差(RSDs)在1.30%~6.64%。将本方法用于实际样品分析,60份婴幼儿食品中黄曲霉毒素B1检出2份,黄曲霉毒素G2检出1份,其余4种黄曲霉毒素均未检出。结论此方法简便快速,可用于婴幼儿食品中6种黄曲霉毒素的测定。
목적：건립료액상색보-천련질보측정영유인식품중6충황곡매독소적방법。방법시양경을정초성제취,리심, MycosepTM226정화주정화후액상색보천련질보의직접측정,외표법정량。결과가표회수솔범위위83.3%~100.6%,상대표준편차(RSDs)재1.30%~6.64%。장본방법용우실제양품분석,60빈영유인식품중황곡매독소B1검출2빈,황곡매독소G2검출1빈,기여4충황곡매독소균미검출。결론차방법간편쾌속,가용우영유인식품중6충황곡매독소적측정。
Objective To establish a method for determination of 6 kinds of aflatoxins in infant food by liquid chromatography-tandem mass spectrometry.Methods Samples were sequentially extracted by acetonitrile, then being centrifuged, using MycosepTM226 columns to cleanup, evaporated, re-dissolved before analysis. Using the external standard method for quantitative analysis, the samples were analysed by liquid chromatography-tandem mass spectrometry.Results The present method showed acceptable recoveries of 83.3%~100.6%, with relative standard deviation (RSDs) ranging from 1.30% to 6.64%. In all 60 samples, 2 samples were detected of aflatoxinB1, and one was detected of aflatoxinG2. In addition, other aflatoxins were not detected in the present study.Conclusion This method is demonstrated to be rapid and simple, which can be applied in the determination of 6 kinds of aflatoxins in infant food.