CAJ | 학술논문

2,3-丁二醇在食品、化妆品、医药和运输燃料等行业具有广泛的应用,因而提高2,3-丁二醇的产量一直备受研究者们关注。目前,研究的热点主要集中于利用微生物发酵可再生资源生产2,3-丁二醇以取代传统的化学合成法,并取得了较大的进展。常见的2,3-丁二醇产生菌有克雷伯氏菌属和芽孢杆菌属,它们能有效利用可再生资源高效生产2,3-丁二醇。然而这些细菌被认为是潜在的病原菌,难以应用于大规模生产。因此,研究者们又将目光转向了酿酒酵母。就安全性和工业化生产要求而言,酿酒酵母是生产2,3-丁二醇的理想菌种。本文对国内外的相关研究进行了总结,介绍了酿酒酵母产2,3-丁二醇的优势和不足,2,3-丁二醇合成代谢途径及其基因工程菌株构建的方向,以及通过代谢工程将酿酒酵母改造成能高效、高质、高量产生2,3-丁二醇的理想菌株的研究关键。
2,3-정이순재식품、화장품、의약화운수연료등행업구유엄범적응용,인이제고2,3-정이순적산량일직비수연구자문관주。목전,연구적열점주요집중우이용미생물발효가재생자원생산2,3-정이순이취대전통적화학합성법,병취득료교대적진전。상견적2,3-정이순산생균유극뢰백씨균속화아포간균속,타문능유효이용가재생자원고효생산2,3-정이순。연이저사세균피인위시잠재적병원균,난이응용우대규모생산。인차,연구자문우장목광전향료양주효모。취안전성화공업화생산요구이언,양주효모시생산2,3-정이순적이상균충。본문대국내외적상관연구진행료총결,개소료양주효모산2,3-정이순적우세화불족,2,3-정이순합성대사도경급기기인공정균주구건적방향,이급통과대사공정장양주효모개조성능고효、고질、고양산생2,3-정이순적이상균주적연구관건。
2,3-Butanediol (2,3-BD) is a compound of significant importance which has an extensive application in the fields of food, cosmetics, medicine and transport fuel. Thus it has being a hot topic about how to improve the 2,3-BD yield in the world. The utilization of renewable resources by microbial fermentation to produce 2,3-BD has been continuously focused on and great advancements have been made by researchers as an alteration of traditional chemical synthesis. Most bacteria such as Klebsiella.sp andBacillus.sp used as 2,3-BD producers can make full use of renewable resources to produce 2,3-BD efficiently. However, most of Klebsiella.sp andBacillus.sp are considered as potential pathogenic which is not suitable for a large-scale production of 2,3-BD. To this end,Saccharomyces cerevisiae is a competent alternative microorganism in terms of the security and the demand for industrial production. This paper reviews the relevant research all over the world. The advantages and disadvantages ofS. cerevisiae as 2,3-BD producer, the metabolism and synthesis routes of 2,3-BD, the directions of enhancing the 2,3-BD yield byS. cerevisiae and key factors to produce 2,3-BD with high efficiency, high quantity and high yield byS. cerevisiaeengineering strains using metabolic engineering are introduced in detail.