CAJ | 학술논문

目的探讨膜联蛋白A2在毛乳头细胞(DPC)凝集生长中的表达特点.方法反转录PCR和Western印迹方法分别在基因和蛋白水平研究膜联蛋白A2在DPC凝集和非凝集生长状态下的表达特点.结果在DPC凝集性生长状态下,膜联蛋白A2mRNA表达水平显著高于非凝集生长状态,差异有统计学意义(0.50±0.15比0.35±0.19,t=8.26,P＜0.05).在蛋白质水平,膜联蛋白A2表现为相对分子质量为40 000的膜联蛋白A2异构体和分子质量为36 000的膜联蛋白A2两种形式,前者在凝集与非凝集生长的DPC均高表达,后者在凝集生长的DPC表达,在非凝集生长的DPC则不表达.结论膜联蛋白A2可能与DPC的凝集生长密切相关.
목적 탐토막련단백A2재모유두세포(DPC)응집생장중적표체특점.방법 반전록PCR화Western인적방법분별재기인화단백수평연구막련단백A2재DPC응집화비응집생장상태하적표체특점.결과 재DPC응집성생장상태하,막련단백A2mRNA표체수평현저고우비응집생장상태,차이유통계학의의(0.50±0.15비0.35±0.19,t=8.26,P＜0.05).재단백질수평,막련단백A2표현위상대분자질량위40 000적막련단백A2이구체화분자질량위36 000적막련단백A2량충형식,전자재응집여비응집생장적DPC균고표체,후자재응집생장적DPC표체,재비응집생장적DPC칙불표체.결론 막련단백A2가능여DPC적응집생장밀절상관.
Objective To analyze the expression characteristics of annexin A2 in dermal papilla cells (DPCs) with aggregative behavior.Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were performed to measure the mRNA and protein expressions of annexin A2 respectively in DPCs with or without aggregative behavior.Results The mRNA expression level of annexin A2 was significantly higher in DPCs with aggregative behavior than in those without aggregative behavior (0.50 ± 0.15 vs.0.35 ± 0.19, t =8.26, P ＜ 0.05).Western blot showed that annexin A2 had two isoforms, including one isoform with a relative molecular mass of 40 000 and the other one with a relative molecular mass of 36 000.The annexin A2 isoform with a relative molecular mass of 40 000 was highly expressed in both DPCs with aggregative behavior and those without aggregative behavior, while the other isoform was only expressed in DPCs with aggregative behavior.Conclusion Annexin A2 may be closely related to the aggregative growth of DPCs.