中国药事
中國藥事
중국약사
Chinese Pharmaceutical Affairs
2015年
10期
1066-1068
,共3页
可见分光光度法%细菌计数%方法研究%枯草芽孢杆菌%短小芽孢杆菌%藤黄微球菌
可見分光光度法%細菌計數%方法研究%枯草芽孢桿菌%短小芽孢桿菌%籐黃微毬菌
가견분광광도법%세균계수%방법연구%고초아포간균%단소아포간균%등황미구균
Vis spectrophotometry%bacterial counts%method study%Bacillus subtilis%Bacillus pumilus%Micrococcus luteus
目的：建立枯草芽孢杆菌、短小芽孢杆菌、藤黄微球菌的快速计数方法。方法：采用可见分光光度法进行细菌计数方法研究，考察菌液浓度与吸光度之间的关系。结果：枯草芽孢杆菌、短小芽孢杆菌、藤黄微球菌菌液的吸光度与其菌液浓度对应在0.68×108~10.88×108、0.80×108~12.80×108、0.89×108~14.30×108浓度范围内线性关系良好（r2分别为0.9999、0.9998、0.9998，n=5），相应菌落计数结果稳定。结论：可见分光光度法可用于3种细菌菌液的快速计数，可以提高药品微生物检定工作效率。
目的：建立枯草芽孢桿菌、短小芽孢桿菌、籐黃微毬菌的快速計數方法。方法：採用可見分光光度法進行細菌計數方法研究，攷察菌液濃度與吸光度之間的關繫。結果：枯草芽孢桿菌、短小芽孢桿菌、籐黃微毬菌菌液的吸光度與其菌液濃度對應在0.68×108~10.88×108、0.80×108~12.80×108、0.89×108~14.30×108濃度範圍內線性關繫良好（r2分彆為0.9999、0.9998、0.9998，n=5），相應菌落計數結果穩定。結論：可見分光光度法可用于3種細菌菌液的快速計數，可以提高藥品微生物檢定工作效率。
목적：건립고초아포간균、단소아포간균、등황미구균적쾌속계수방법。방법：채용가견분광광도법진행세균계수방법연구，고찰균액농도여흡광도지간적관계。결과：고초아포간균、단소아포간균、등황미구균균액적흡광도여기균액농도대응재0.68×108~10.88×108、0.80×108~12.80×108、0.89×108~14.30×108농도범위내선성관계량호（r2분별위0.9999、0.9998、0.9998，n=5），상응균락계수결과은정。결론：가견분광광도법가용우3충세균균액적쾌속계수，가이제고약품미생물검정공작효솔。
Objective:To establish rapid bacterial counting methods forBacillus subtilis, Bacillus pumilusand Micrococcus luteus.Methods: Vis spectrophotometry was employed for bacterial counts. The correlation between absorbance and concentration of bacteria solution was investigated.Results:The absorbance and the concentration of bacteria solution had good linear relationship (r2=0.9999, 0.9998, 0.9998,n=5) when the absorbance and of Bacillus subtilis solution,Bacillus pumilus solution andMicrococcus luteus solution were respectively between 0.68×108~10.88 ×108, 0.80×108~12.80×108, 0.89×108~14.30×108. The results of bacterial counts were stable. Conclusion:Vis spectrophotometry is suitable in the three kinds of bacteria counting, which can signiifcantly improve the working efifciency in medicine microbial tests.
