CAJ | 학술논문

目的检测沙眼衣原体临床株对利福平的体外敏感性,探讨rpoB基因突变与临床耐药的关系.方法采用微量细胞培养法确定利福平对52株沙眼衣原体临床株的最低抑菌浓度.扩增所有临床株以及标准株rpoB基因,然后进行单链构象多态性分析.并随机选取两株临床株进行测序.结果 52株临床菌株中未检出耐药株,利福平的最低抑菌浓度是0.004 ～ 0.030 mg/L.SSCP和测序均未发现rpoB耐药突变.结论利福平治疗沙眼衣原体失败患者未检测到rpoB基因突变,利福平治疗失败与多种因素有关.
목적 검측사안의원체림상주대리복평적체외민감성,탐토rpoB기인돌변여림상내약적관계.방법 채용미량세포배양법학정리복평대52주사안의원체림상주적최저억균농도.확증소유림상주이급표준주rpoB기인,연후진행단련구상다태성분석.병수궤선취량주림상주진행측서.결과 52주림상균주중미검출내약주,리복평적최저억균농도시0.004 ～ 0.030 mg/L.SSCP화측서균미발현rpoB내약돌변.결론 리복평치료사안의원체실패환자미검측도rpoB기인돌변,리복평치료실패여다충인소유관.
Objective To evaluate the susceptibility of Chlamydia trachomatis clinical isolates to rifampin, and assess the relationship between rpoB mutations and antibiotic resistance in them.Methods A microculture method was used to determine the minimal inhibitory concentration (MIC) of rifampin in 52 Chlamydia trachomatis clinical isolates.The rpoB gene was amplified from all the clinical isolates and a standard strain of Chlamydia trachomatis followed by single-strand conformation polymorphism (SSCP)analysis.Sequencing of PCR products was carried out for two clinical isolates.Results No rifampin-resistant strain was found among these clinical isolates.The MIC of rifampin varied from 0.004 to 0.030 mg/L Neither SSCP analysis nor sequencing showed rpoB mutations.Conclusions No rpoB mutations were found in Chlamydia trachomatis isolates from patients unresponsive to rifampin.The unresponsiveness to rifampin may be attributed to multiple factors.