中华眼外伤职业眼病杂志
中華眼外傷職業眼病雜誌
중화안외상직업안병잡지
Chinese Journal of ocular trauma and occupational eye disease
2015年
10期
739-743
,共5页
脉络膜%新生血管形成%血管抑制因子( Vasostatin)%疾病模型,小鼠
脈絡膜%新生血管形成%血管抑製因子( Vasostatin)%疾病模型,小鼠
맥락막%신생혈관형성%혈관억제인자( Vasostatin)%질병모형,소서
Choroidal neovascularization%Vasostatin%Models,disease,mice
目的：探讨重组人血管抑制因子( vasostatin)抑制激光诱导的小鼠脉络膜新生血管形成(CNV)的作用。方法 C57BL/6J成年小鼠30只(30只眼),均用右眼,通过Nd： YAG激光眼底光凝诱导小鼠形成脉络膜新生血管,随机分为对照组、低剂量组和高剂量组,每组各10只小鼠。在眼底Nd：YAG激光光凝后立即分别给予A组(对照组)每只小鼠球后注射10μl生理盐水；B组(低剂量组)各组每只小鼠球后注射血管抑制因子10μl(2μg/μl)；C组(高剂量组)注射10μl(4μg/μl)。在光凝后7 d及14 d通过荧光素眼底血管造影( FFA)评价CNV的发生率。结果治疗组在光凝后7 d及14 d CNV发生率及各光凝斑荧光素渗漏程度均比对照组轻。光凝后7 d,A组脉络膜新生血管的发生率为68%,B组和C组脉络膜新生血管的发生率分别为56%、48%,与A组比较,差异均有统计学意义(P<0.05)。光凝后14 d,A组脉络膜新生血管的发生率为72%,B组和C组脉络膜新生血管的发生率分别为50%、38%,与A组比较,差异均有统计学意义(P<0.01)。结论血管抑制因子可以有效抑制激光诱导的小鼠实验性CNV。通过Nd：YAG激光照射脉络膜损伤成功地诱导了小鼠CNV模型,这种CNV模型制作方法可用于CNV的发病机制及治疗方法的研究。
目的：探討重組人血管抑製因子( vasostatin)抑製激光誘導的小鼠脈絡膜新生血管形成(CNV)的作用。方法 C57BL/6J成年小鼠30隻(30隻眼),均用右眼,通過Nd： YAG激光眼底光凝誘導小鼠形成脈絡膜新生血管,隨機分為對照組、低劑量組和高劑量組,每組各10隻小鼠。在眼底Nd：YAG激光光凝後立即分彆給予A組(對照組)每隻小鼠毬後註射10μl生理鹽水；B組(低劑量組)各組每隻小鼠毬後註射血管抑製因子10μl(2μg/μl)；C組(高劑量組)註射10μl(4μg/μl)。在光凝後7 d及14 d通過熒光素眼底血管造影( FFA)評價CNV的髮生率。結果治療組在光凝後7 d及14 d CNV髮生率及各光凝斑熒光素滲漏程度均比對照組輕。光凝後7 d,A組脈絡膜新生血管的髮生率為68%,B組和C組脈絡膜新生血管的髮生率分彆為56%、48%,與A組比較,差異均有統計學意義(P<0.05)。光凝後14 d,A組脈絡膜新生血管的髮生率為72%,B組和C組脈絡膜新生血管的髮生率分彆為50%、38%,與A組比較,差異均有統計學意義(P<0.01)。結論血管抑製因子可以有效抑製激光誘導的小鼠實驗性CNV。通過Nd：YAG激光照射脈絡膜損傷成功地誘導瞭小鼠CNV模型,這種CNV模型製作方法可用于CNV的髮病機製及治療方法的研究。
목적：탐토중조인혈관억제인자( vasostatin)억제격광유도적소서맥락막신생혈관형성(CNV)적작용。방법 C57BL/6J성년소서30지(30지안),균용우안,통과Nd： YAG격광안저광응유도소서형성맥락막신생혈관,수궤분위대조조、저제량조화고제량조,매조각10지소서。재안저Nd：YAG격광광응후립즉분별급여A조(대조조)매지소서구후주사10μl생리염수；B조(저제량조)각조매지소서구후주사혈관억제인자10μl(2μg/μl)；C조(고제량조)주사10μl(4μg/μl)。재광응후7 d급14 d통과형광소안저혈관조영( FFA)평개CNV적발생솔。결과치료조재광응후7 d급14 d CNV발생솔급각광응반형광소삼루정도균비대조조경。광응후7 d,A조맥락막신생혈관적발생솔위68%,B조화C조맥락막신생혈관적발생솔분별위56%、48%,여A조비교,차이균유통계학의의(P<0.05)。광응후14 d,A조맥락막신생혈관적발생솔위72%,B조화C조맥락막신생혈관적발생솔분별위50%、38%,여A조비교,차이균유통계학의의(P<0.01)。결론혈관억제인자가이유효억제격광유도적소서실험성CNV。통과Nd：YAG격광조사맥락막손상성공지유도료소서CNV모형,저충CNV모형제작방법가용우CNV적발병궤제급치료방법적연구。
Objective To investigate the effects of recombinant human Vasostatin on the inhibition of Nd:YAG laser induced mice choroidal neovascularization (CNV). Methods In 30 adult C57BL/6J mice(30 eyes), choroid angiogenesis was induced by Nd: YAG laser fundus photocoagulation in the right eye. The mice were randomly divided into control group, low dose group and high dose group with 10 mice in each group. Immediately after the Nd:YAG laser photocoagulation, retrobulbar injection of 10 μl saline was peformed in all mice in group A ( control group);Mice in treatment group was retrobulbarly injected with 10μl of recombinant human Vasostatin at the concentration of 2μg/μl in group B and 4μg/μl in group C. The choroidal neovascularization was evaluated by fundus fluorescence angiography 7 and 14 days after photocoagulation. Results In the treatment group, the incidence of CNV and spot fluorescein leakage were lighter than that of control group 7 d and 14 d after photocoagulation. Seven days after photocoagulation, choroidal neovascularization incidence was 68% in group A. The incidence rates of choroid neovascularization in group B and group C were 56% and 48% respectively. There was statistically significant difference (P<0. 05). Fourteen days after photocoagulation, the choroid neovascularization incidence was 72% in group A. It was 50% and 38% in group B and group C respectively, which was statistically significant different from group A (P<0. 01). Conclusion Vasostatin can effectively suppress experimental laser-induced mouse CNV. Choroidal injury by the Nd:YAG laser can successfully induce mouse CNV model.
