CAJ | 학술논문

目的：建立跌打丸中非法染色剂808猩红的检测方法。方法：采用高效液相色谱法，色谱柱为Agilent Eclipse Plus C18柱，柱温30℃，体积流量1.0 mL·min-1，流动相为乙腈-0.02 mol·L-1醋酸铵溶液，梯度洗脱，检测波长516 nm。液质联用验证采用Waters Acquity UPLC BEH C18柱，流动相为乙腈-5 mmol·L-1醋酸铵溶液，梯度洗脱，ESI+扫描模式。供试样品中相关分子离子峰及碎片特征与808猩红对照试剂一致。结果：含量测定808猩红在0.01136~0.11360μg范围内呈良好的线性关系。平均加样回收率为96.95%，RSD为1.14%。结论：该方法简便、快速、准确，专属性、稳定性、重复性良好。
목적：건립질타환중비법염색제808성홍적검측방법。방법：채용고효액상색보법，색보주위Agilent Eclipse Plus C18주，주온30℃，체적류량1.0 mL·min-1，류동상위을정-0.02 mol·L-1작산안용액，제도세탈，검측파장516 nm。액질련용험증채용Waters Acquity UPLC BEH C18주，류동상위을정-5 mmol·L-1작산안용액，제도세탈，ESI+소묘모식。공시양품중상관분자리자봉급쇄편특정여808성홍대조시제일치。결과：함량측정808성홍재0.01136~0.11360μg범위내정량호적선성관계。평균가양회수솔위96.95%，RSD위1.14%。결론：해방법간편、쾌속、준학，전속성、은정성、중복성량호。
Objective: To establish a method for the detection of illegally added 808 Scarlet in Dieda Pills. Methods:Applying the method of HPLC to an Agilent Eclipse Plus C18 column, 808 Scarlet was separated by using acetonitrile-0.02 mol·L-1 ammonium acetate as the mobile phase under gradient elution. The UV detector was set at 516 nm; the column temperature was at 30℃ and the lfow rate was 1.0 mL·min-1. The method of LC-MS was conducted on a Waters Acquity UPLC BEH C18 column; the mobile phase was composed of acetonitrile and 5 mmol·L-1 ammonium acetate under gradient elution in the ESI+ scanning mode. The ion peaks of related molecules in test samples and fragment features were identical to the reference reagent of 808 Scarlet.Results:808 Scarlet showed good linearity within 0.01136-0.11360 μg and the average recovery was 96.95% (RSD=1.14%). Conclusion:The method is simple, rapid and accurate with good speciifcity, stability and repeatability.