中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2015年
10期
687-691
,共5页
张丽君%王峰%莫俊銮%彭毅
張麗君%王峰%莫俊鑾%彭毅
장려군%왕봉%막준란%팽의
淋病奈瑟球菌%头孢曲松%DNA突变分析%微生物敏感性试验%抗药性,微生物
淋病奈瑟毬菌%頭孢麯鬆%DNA突變分析%微生物敏感性試驗%抗藥性,微生物
임병내슬구균%두포곡송%DNA돌변분석%미생물민감성시험%항약성,미생물
Neisseria gonorrhoeae%Ceftriaxone%DNA mutational analysis%Microbial sensitivity tests%Drug resistance,microbial
目的 了解penA、ponA、porB和mtrR突变与深圳市淋球菌对头孢曲松敏感性降低的相关性.方法 收集2009-2011年深圳市淋球菌临床分离株296株,采用琼脂稀释法筛选出头孢曲松低敏株[MIC(0.06 ～ 0.50) μg/ml]53株.将头孢曲松低敏菌株以及按照1:1抽样原则随机抽取的53株高敏菌株,共计106株淋球菌作为试验菌株.对所有菌株penA、ponA、porB和mtrR基因进行PCR扩增以及DNA测序分析.结果 1株淋球菌的青霉素结合蛋白2(penicillin-binding protein 2,PBP2,由penA基因编码)具有镶嵌样结构(MIC0.125 0 μg/ml),对剩余105株淋球菌PBP2的氨基酸序列分析,共得到16个不同的氨基酸模式.模式Ⅻ、ⅩⅢ、ⅩⅩⅩⅧ对应的头孢曲松MIC值相对较高(MIC50均为0.062 5 μg/ml),而模式Ⅱ的头孢曲松MIC值相对较低(MIC50为0.008 0μg/ml).mtrR、porB以及ponA突变在头孢曲松低敏组和高敏组中的发生率,差异无统计学意义(均P＞ 0.05).结论 PBP2镶嵌样结构可能不是深圳市淋球菌对头孢曲松敏感性降低的主要原因,非镶嵌样PBP2 500 ～ 580位多个氨基酸突变产生的不同氨基酸模式联合mtrR、 porB以及ponA突变在诱导淋球菌对头孢曲松敏感性降低中可能有意义.
目的 瞭解penA、ponA、porB和mtrR突變與深圳市淋毬菌對頭孢麯鬆敏感性降低的相關性.方法 收集2009-2011年深圳市淋毬菌臨床分離株296株,採用瓊脂稀釋法篩選齣頭孢麯鬆低敏株[MIC(0.06 ～ 0.50) μg/ml]53株.將頭孢麯鬆低敏菌株以及按照1:1抽樣原則隨機抽取的53株高敏菌株,共計106株淋毬菌作為試驗菌株.對所有菌株penA、ponA、porB和mtrR基因進行PCR擴增以及DNA測序分析.結果 1株淋毬菌的青黴素結閤蛋白2(penicillin-binding protein 2,PBP2,由penA基因編碼)具有鑲嵌樣結構(MIC0.125 0 μg/ml),對剩餘105株淋毬菌PBP2的氨基痠序列分析,共得到16箇不同的氨基痠模式.模式Ⅻ、ⅩⅢ、ⅩⅩⅩⅧ對應的頭孢麯鬆MIC值相對較高(MIC50均為0.062 5 μg/ml),而模式Ⅱ的頭孢麯鬆MIC值相對較低(MIC50為0.008 0μg/ml).mtrR、porB以及ponA突變在頭孢麯鬆低敏組和高敏組中的髮生率,差異無統計學意義(均P＞ 0.05).結論 PBP2鑲嵌樣結構可能不是深圳市淋毬菌對頭孢麯鬆敏感性降低的主要原因,非鑲嵌樣PBP2 500 ～ 580位多箇氨基痠突變產生的不同氨基痠模式聯閤mtrR、 porB以及ponA突變在誘導淋毬菌對頭孢麯鬆敏感性降低中可能有意義.
목적 료해penA、ponA、porB화mtrR돌변여심수시림구균대두포곡송민감성강저적상관성.방법 수집2009-2011년심수시림구균림상분리주296주,채용경지희석법사선출두포곡송저민주[MIC(0.06 ～ 0.50) μg/ml]53주.장두포곡송저민균주이급안조1:1추양원칙수궤추취적53주고민균주,공계106주림구균작위시험균주.대소유균주penA、ponA、porB화mtrR기인진행PCR확증이급DNA측서분석.결과 1주림구균적청매소결합단백2(penicillin-binding protein 2,PBP2,유penA기인편마)구유양감양결구(MIC0.125 0 μg/ml),대잉여105주림구균PBP2적안기산서렬분석,공득도16개불동적안기산모식.모식Ⅻ、ⅩⅢ、ⅩⅩⅩⅧ대응적두포곡송MIC치상대교고(MIC50균위0.062 5 μg/ml),이모식Ⅱ적두포곡송MIC치상대교저(MIC50위0.008 0μg/ml).mtrR、porB이급ponA돌변재두포곡송저민조화고민조중적발생솔,차이무통계학의의(균P＞ 0.05).결론 PBP2양감양결구가능불시심수시림구균대두포곡송민감성강저적주요원인,비양감양PBP2 500 ～ 580위다개안기산돌변산생적불동안기산모식연합mtrR、 porB이급ponA돌변재유도림구균대두포곡송민감성강저중가능유의의.
Objective To analyze the relationship of penA, ponA, porB and mtrR gene mutations with the reduced sensitivity to ceftriaxone in N.gonorrhoeae isolates from Shenzhen city.Methods A total of 296 clinical isolates of N.gonorrhoeae were collected in Shenzhen city from 2009 to 2011.The agar dilution method was used to estimate the sensitivity of these N.gonorrhoeae to ceftriaxone.Totally, 53 strains with reduced sensitivity to ceftriaxone (minimum inhibitory concentration (MIC): 0.06-0.50 μg/ml) were identified, and 53 strains with high sensitivity to ceftriaxone were randomly selected from the remaining strains and served as the control group.PCR was performed to amplify the penA, ponA,porB and mtrR genes from the 106 isolates followed DNA sequencing.Results The mosaic structure of the penicillinbinding protein 2 (PBP2) gene (penA gene) was found in only one isolate with a ceftriaxone MIC of 0.125 0 μg/ml.Amino acid sequence analysis of the remaining 105 isolates yielded 16 different amino acid patterns.The MICs of ceftriaxone were relatively high (0.062 5 μg/ml) in N.gonorrhoeae strains harboring the amino acid patterns ⅩⅢ, ⅩⅧ or ⅩⅩⅩⅧ,but relatively low (0.008 0 μg/ml) in those harboring the amino acid pattern Ⅱ.No significant differences were observed in the frequency of mtrR, porB or ponA gene mutations between N.gonorrhoeae isolates with reduced sensitivity to ceftriaxone and those with high sensitivity (all P ＞ 0.05).Conclusions The mosaic structure of PBP2 may be not the primary reason for reduced sensitivity of Neisseria gonorrhoeae to ceftriaxone in Shenzhen, while different amino acid patterns produced by various mutations in amino acid residues at positions 500-580 in the non-mosaic PBP2, together with mtrR, porB and ponA mutations, may play more important roles in the reduced sensitivity.
