CAJ | 학술논문

目的:探讨CDH17表达与胃癌临床病理学特征的关系及其SNPs与胃癌遗传易感性的关系.方法:制作模拟胃癌侵袭转移动态的组织芯片,免疫组织化学SP法检测CDH17蛋白的表达,PCR-LDR技术检测CDH17基因2个SNPs位点rs2514813和rs3214050的多态性并行测序验证.结果:胃癌CDH17表达上调与组织学分化程度呈正相关(P<0.01);肠型胃癌表达高于弥漫型胃癌;随着侵袭过程的进展,黏膜层、肿瘤中央区、侵袭前沿区胃癌的CDH17表达水平呈下降趋势;CDH17表达与胃癌术后生存时间未见明显相关(P=0.209).胃癌CDH17基因SNP位点rs3214050等位基因C、T频率及CC、CT和TT的基因型频率与对照组相比差异有统计学意义(P<0.01),携带等位基因T的胃癌患者术后生存时间长于CC基因型(P<0.01).rs2514813位点基因型与等位基因频率与对照组相比无显著性差异(P>0.05).结论:CDH17表达升高与胃癌腺样结构的维持及进展相关,rs3214050位点为T等位基因者患胃癌风险低且预后好,提示该位点与中国福建汉族人群胃癌的遗传易感性及预后有关.
목적:탐토CDH17표체여위암림상병이학특정적관계급기SNPs여위암유전역감성적관계.방법:제작모의위암침습전이동태적조직심편,면역조직화학SP법검측CDH17단백적표체,PCR-LDR기술검측CDH17기인2개SNPs위점rs2514813화rs3214050적다태성병행측서험증.결과:위암CDH17표체상조여조직학분화정도정정상관(P<0.01);장형위암표체고우미만형위암;수착침습과정적진전,점막층、종류중앙구、침습전연구위암적CDH17표체수평정하강추세;CDH17표체여위암술후생존시간미견명현상관(P=0.209).위암CDH17기인SNP위점rs3214050등위기인C、T빈솔급CC、CT화TT적기인형빈솔여대조조상비차이유통계학의의(P<0.01),휴대등위기인T적위암환자술후생존시간장우CC기인형(P<0.01).rs2514813위점기인형여등위기인빈솔여대조조상비무현저성차이(P>0.05).결론:CDH17표체승고여위암선양결구적유지급진전상관,rs3214050위점위T등위기인자환위암풍험저차예후호,제시해위점여중국복건한족인군위암적유전역감성급예후유관.
Objective:To explore the relationship of CDH17 expression with clinico-pathological features and the correlation be-tween the single nucleotide polymorphisms (SNPs) of CDH17 gene and genetic susceptibility of gastric carcinoma (GC). Methods:A tissue microarray was performed to simulate the dynamic process of invasion and metastasis of GC. Immunohistochemical staining was performed to detect the expression of CDH17 protein, and PCR-based LDR was performed to detect the 2 SNP loci (rs2514813 and rs3214050) genotypes of CDH17 gene. Results: The expression of CDH17 protein in GC was more significantly up-regulated and greatly increased in the intestinal type than in the diffuse type. The expression of CDH17 protein in GC was positively correlated with the histological grading (P<0.01) and was not associated with the survival (P=0.209). With the progression of the cancer invasion, the expression of CDH17 protein in GC showed a downtrend from the gastric mucosa layer to the invasive front edge. The frequencies of the C and T alleles and the CC, CT, and TT genotypes at the CDH17 rs3214050 locus between the GC patients and the control groups were significantly different (P<0.01). However, no significant differences were observed at rs2514813 (P>0.05). The individuals with the T al-leles had longer survival time than those with the CC genotype (P<0.01). Conclusion:The up-regulation of CDH17 expression is in-volved in the maintenance of histological phenotype and progression of GC. Individuals with T alleles at the CDH17 rs3214050 locus have decreased risk of GC and had better prognosis (OR=0.762, 95%CI:0.619-0.937), thereby suggesting that screening for these alleles would help with the assessment of genetic susceptibility and prognosis of GC in the Fujian population.