绍兴文理学院学报
紹興文理學院學報
소흥문이학원학보
Journal of Shaoxing University
2015年
9期
7-9,14
,共4页
载体%腈水解酶%固定化%酶活
載體%腈水解酶%固定化%酶活
재체%정수해매%고정화%매활
carrier%nitrilase%immobilization%activity
研究磁性纳米载体的氨基化修饰及其固定化腈水解酶的条件,探讨固定化腈水解酶的酶学性能.研究结果表明载体固定腈水解酶的最佳工艺为:腈水解酶与载体配比15mg/g、戊二醛浓度3% 、固定化温度30℃、固定化时间60min.固定化腈水解酶的热稳定性及pH稳定性都比游离酶有很大提高.
研究磁性納米載體的氨基化脩飾及其固定化腈水解酶的條件,探討固定化腈水解酶的酶學性能.研究結果錶明載體固定腈水解酶的最佳工藝為:腈水解酶與載體配比15mg/g、戊二醛濃度3% 、固定化溫度30℃、固定化時間60min.固定化腈水解酶的熱穩定性及pH穩定性都比遊離酶有很大提高.
연구자성납미재체적안기화수식급기고정화정수해매적조건,탐토고정화정수해매적매학성능.연구결과표명재체고정정수해매적최가공예위:정수해매여재체배비15mg/g、무이철농도3% 、고정화온도30℃、고정화시간60min.고정화정수해매적열은정성급pH은정성도비유리매유흔대제고.
Amination modification of ferriferous oxide magnetic nanoparticles was studied. The conditions of im-mobiling nitrilase by APTES-coated Fe3O4 nanoparticles were also researched. The results show that the optimal factors of immobilized nitrilase are as follows:The mass ratio of enzyme and carrier is 5mg/g, temperature 30℃, time 60 min and the glutaraldehyde 3%. Compared with the free nitrilase, the thermal stability, pH stability and operational stability of the immobilized enzyme are all greatly improved.