CAJ | 학술논문

目的：观察热疗联合阿霉素对人多发性骨髓瘤细胞株ARH-77细胞内的药物浓度变化及凋亡的影响。方法 MTT法确定阿霉素的工作浓度，以该浓度进行化疗或与热疗的联合，选择温度40℃、41℃及42℃，体外作用于ARH-77细胞。作用前及48 h采用台盼蓝拒染法检测肿瘤细胞的存活率；MTT法检测肿瘤细胞增殖的抑制作用；流式细胞仪检测肿瘤细胞的凋亡及细胞内药物浓度的变化。观察热疗联合阿霉素的抗肿瘤效果。结果作用48h IC50的药物浓度作为实验的工作浓度。热化疗组对ARH-77细胞有明显的抑制作用（ P﹤0.01），随着温度的增高而增强；热化疗组细胞内的药物浓度明显增加（ P﹤0.01）。结论热疗联合阿霉素能增加ARH-77细胞内的药物浓度，提高肿瘤细胞的凋亡率。
목적：관찰열료연합아매소대인다발성골수류세포주ARH-77세포내적약물농도변화급조망적영향。방법 MTT법학정아매소적공작농도，이해농도진행화료혹여열료적연합，선택온도40℃、41℃급42℃，체외작용우ARH-77세포。작용전급48 h채용태반람거염법검측종류세포적존활솔；MTT법검측종류세포증식적억제작용；류식세포의검측종류세포적조망급세포내약물농도적변화。관찰열료연합아매소적항종류효과。결과작용48h IC50적약물농도작위실험적공작농도。열화료조대ARH-77세포유명현적억제작용（ P﹤0.01），수착온도적증고이증강；열화료조세포내적약물농도명현증가（ P﹤0.01）。결론열료연합아매소능증가ARH-77세포내적약물농도，제고종류세포적조망솔。
Objective:To observe the effect of thermotherapy on the intracellular adriamycin concentration and apoptosis of ARH-77 cells in vitro. Methods:The working concentration of adriamycin against ARH-77 was determined by MTT as-say. ARH-77 cells were subjected to thermotherapy( at 40℃,41℃,42℃)and chemotherapy with adriamycin alone or in conjunction,and the cell survival rates were determined at 48h after the treatment. The cell growth inhibition effect of the treatment was evaluated with MTT assay,and the apoptosis rates of ARH-77 and alteration of intracellular adriamycin con-centration were determined by flow cytometric analyses. Results:The IC50 of adriamycin was defined as the working con-centration in the experiment. The thermotherapy at 40,41 and 42℃ for 60 min in conjunction with chemotherapy signifi-cantly inhibited the growth of ARH-77 cells(P﹤0. 01). The results of flow cytometric analyses showed that thermotherapy and adriamycin chemotherapy,used either alone or in conjunction,obviously increased the apoptosis rates of ARH-77 cells ( P﹤0. 01)and thermotherapy remarkably increased the intracellular concentration of adriamycin. Conclusion:Adriamycin chemotherapy combined thermotherapy for 60min can increase the intracellular concentration of adriamycin and the apopto-sis rates of ARH-77 cells.