泰山医学院学报
泰山醫學院學報
태산의학원학보
Journal of Taishan Medical College
2015年
9期
982-984
,共3页
正天丸%戊巴比妥钠%巴比妥钠%催眠作用%细胞色素P450
正天汍%戊巴比妥鈉%巴比妥鈉%催眠作用%細胞色素P450
정천환%무파비타납%파비타납%최면작용%세포색소P450
Zheng-tian pill%pentobarbital sodium%barbital sodium%hypnotic effect%cytochrome P450
目的：通过观察正天丸对戊巴比妥钠及巴比妥钠催眠作用的影响，了解正天丸对肝微粒体细胞色素P450活性的影响。方法将60只小鼠随机分为溶媒组（2%吐温-80，20 ml/kg）、氯霉素组（25 mg/kg）及正天丸低、高剂量组（3，6 g/kg），每组15只，连续等容灌胃3d，末次给药1h后，再给小鼠腹腔注射戊巴比妥钠40 mg/kg或巴比妥钠180 mg/kg，观察小鼠的睡眠潜伏期和睡眠时间。结果氯霉素组和低、高剂量的正天丸组使戊巴比妥钠对小鼠的催眠时间分别延长222.2%、123.1%、42.6%，其中氯霉素组和小剂量正天丸组与溶媒组比较，小鼠的睡眠时间显著延长，均P﹤0.01。与溶媒组比较，高剂量的正天丸能显著延长巴比妥钠催眠潜伏期（ P﹤0.01），并缩短催眠时间（ P﹤0.01）。结论正天丸可能抑制小鼠肝微粒体细胞色素P450活性，同时对中枢神经系统有兴奋作用。
目的：通過觀察正天汍對戊巴比妥鈉及巴比妥鈉催眠作用的影響，瞭解正天汍對肝微粒體細胞色素P450活性的影響。方法將60隻小鼠隨機分為溶媒組（2%吐溫-80，20 ml/kg）、氯黴素組（25 mg/kg）及正天汍低、高劑量組（3，6 g/kg），每組15隻，連續等容灌胃3d，末次給藥1h後，再給小鼠腹腔註射戊巴比妥鈉40 mg/kg或巴比妥鈉180 mg/kg，觀察小鼠的睡眠潛伏期和睡眠時間。結果氯黴素組和低、高劑量的正天汍組使戊巴比妥鈉對小鼠的催眠時間分彆延長222.2%、123.1%、42.6%，其中氯黴素組和小劑量正天汍組與溶媒組比較，小鼠的睡眠時間顯著延長，均P﹤0.01。與溶媒組比較，高劑量的正天汍能顯著延長巴比妥鈉催眠潛伏期（ P﹤0.01），併縮短催眠時間（ P﹤0.01）。結論正天汍可能抑製小鼠肝微粒體細胞色素P450活性，同時對中樞神經繫統有興奮作用。
목적：통과관찰정천환대무파비타납급파비타납최면작용적영향，료해정천환대간미립체세포색소P450활성적영향。방법장60지소서수궤분위용매조（2%토온-80，20 ml/kg）、록매소조（25 mg/kg）급정천환저、고제량조（3，6 g/kg），매조15지，련속등용관위3d，말차급약1h후，재급소서복강주사무파비타납40 mg/kg혹파비타납180 mg/kg，관찰소서적수면잠복기화수면시간。결과록매소조화저、고제량적정천환조사무파비타납대소서적최면시간분별연장222.2%、123.1%、42.6%，기중록매소조화소제량정천환조여용매조비교，소서적수면시간현저연장，균P﹤0.01。여용매조비교，고제량적정천환능현저연장파비타납최면잠복기（ P﹤0.01），병축단최면시간（ P﹤0.01）。결론정천환가능억제소서간미립체세포색소P450활성，동시대중추신경계통유흥강작용。
Objective:To observe the influence of Zheng-tian pill on the hypnotic effects of pentobarbital sodium and barbital sodium. Methods:Mice were randomly divided into solvent(5% Tween-80,20 ml/kg),chloromycetin(25 mg/kg)and Zheng-tian pill(3,6 g/kg)groups,which were administered orally with equivalent volume,once a day and for three days. An hour later at the last administration,all the animals were given pentobarbital sodium(40 mg/kg,ip)or bar-bital sodium(180 mg/kg,ip),and then the latent period and duration of hypnosis were observed. Results:Hypnotic dura-tion of pentobarbital sodium was prolonged by 222. 2%,123. 1% and 42. 6% in chloromycetin and two Zheng-tian pill groups respectively,chloromycetin group and Zheng-tian pill group at the lower dose compared with solvent group,there were evident difference(P﹤0. 01),and yet the large dose of Zheng-tian pill prolonged evidently hypnotic latent period(P﹤0. 01)and shortened obviously hypnotic duration(P﹤0. 01)of barbital sodium. Conclusion:The findings in this study suggest that Zheng-tian pill is likely to inhibit liver microsomal cytochrome P450 activity and to excite the central nervous system at a higher dose in mice.
